beta Tubulin Antibody (SAP.4G5)

Immunohistochemistry-Paraffin: beta Tubulin Antibody (SAP.4G5) [NB120-11312]

Immunohistochemistry-Paraffin: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - Staining of formalin-fixed, paraffin-embedded human fallopian tube sections with Monoclonal Anti-beta-Tubulin I, Clone: SAP.4G5 using biotin/ExtrAvidin(R)-Peroxidase.

Western Blot: beta Tubulin Antibody (SAP.4G5) [NB120-11312] -

Characterization of p2TK2-SW2-Tet-J-E-hctB-flag.A) Schematic of the Te-riboJ-E-hctB-flag construct. HctB expression is controlled by an aTc inducible promoter, riboJ insulator and the riboE riboswitch. B) Anti-flag western blot of Cos-7 cells infected with L2-Tet-J-E-hctB-flag comparing expression of theophylline treated and untreated cultures. Cells were induced with 0.5 mM theophylline, 30ng/ml aTc, both aTc and theophylline or vehicle only at 16 hpi and proteins were harvested at 30 hpi. HctB-flag expression was only detected in the samples induced with both aTc and theophylline. C) Confocal micrographs of Cos-7 cells infected with L2-Tet-riboJ-E-hctB-flag, induced with 0.5 mM theophylline, 30ng/ml aTc, both aTc and theophylline or vehicle only at 16 hpi and fixed and stained with DAPI (blue) for confocal microscopy at 30 hpi. The flag tag was stained with a primary antibody to the flag and an alexa 488 anti-mouse secondary antibody (green). Size bar = 10 μm. D) Production of infectious progeny was determined using a reinfection assay. Cos-7 cells were infected with L2-Tet-J-E-hctB-flag and the production of infectious progeny was determined at 48 hpi after induction with 0.5 mM theophylline, 30ng/ml aTc, both aTc and theophylline or vehicle only at 16 hpi. Asterisks denote p-values < 0.05. Error bars = SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35085261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: beta Tubulin Antibody (SAP.4G5) [NB120-11312] -

Characterization of p2TK2-SW2-nprom-E-pgp4-flag.A) Schematic of the nprom-E-pgp4-flag construct consisting of the native pgp4 promoter, the riboE riboswitch (rsE), and the pgp4 ORF with an inframe 3x flag tag. B) Anti-flag western blot of Cos-7 cells infected with L2-nprom-E-pgp4-flag comparing expression of theophylline treated and untreated cultures. Cells were induced or not with 0.5mM theophylline at 16 hpi and proteins were harvested at 30 hpi. A beta -tubulin I western blot served as a loading control. C) Confocal micrographs of Cos-7 cells infected with L2-nprom-E-pgp4-flag, induced or not with 0.5 mM theophylline at 16 hpi and fixed and stained with DAPI to detect DNA. The flag tag was detected using a primary antibody to the tag and an alexa 488 anti-mouse secondary antibody (green). Size bar = 10 μm. D) Cos-7 cells were infected with L2-nprom-E-pgp4-flag and the production of infectious progeny was determined at 48 hpi after 0.5 mM theophylline induction or vehicle only. E) Iodine staining of glycogen in the inclusion of Cos-7 cells infected with L2-nprom-E-pgp4-flag after 0.5 mM theophylline induction at 16 hpi or vehicle only. Arrows indicate the location of the chlamydial inclusions. Asterisk denotes p-value < 0.05. Error bars = SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35085261), licensed under a CC-BY license. Not internally tested by Novus Biologicals.