BLIMP1/PRDM1 Antibody

Novus Biologicals | Catalog # NBP1-51991

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BLIMP1/PRDM1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DISDNADRLEDVED, from the internal region of the protein sequence according to NP_001189.2; NP_878911.1.

Epitope

C-DISDNADRLEDVED

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32981181)

Marker

Plasma Cell Marker

Specificity

This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BLIMP1/PRDM1 Antibody

Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991]

Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991]

Western Blot: BLIMP1/PRDM1 Antibody [NBP1-51991] - Staining of A431 (A), (2 ug/mL) K562 (B) and (1 ug/mL) Nuclear K562 (C) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991]

Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991]

Flow Cytometry: BLIMP1/PRDM1 Antibody [NBP1-51991] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991]

Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991]

Immunofluorescence: BLIMP1/PRDM1 Antibody [NBP1-51991] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
BLIMP1/PRDM1 Antibody

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] -

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
BLIMP1/PRDM1 Antibody

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] -

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP1-51991] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

Applications for BLIMP1/PRDM1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry-Paraffin

6 ug/ml

Peptide ELISA

Detection limit 1:1000

Western Blot

0.1 - 2 ug/mL
Application Notes
WB: Approx 90kDa band observed in lysates of cell lines A431 and Nuclear K562, and approx. 100kDa in lysates of cell line K562. An additional faint band of approx 70kDa was also seen in some A431 and K562 lysates (calculated MW of 91.8kDa according to NP_001189.2 isoform 1 and 76.8kD according to NP_878911.1 isoform 2). Primary incubation 1 hour at room temperature.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BLIMP1/PRDM1

Blimp-1 (B lymphocyte maturation protein-1) is a transcriptional repressor that is required to trigger terminal differentiation of B lymphocytes into Ig secreting cells. Therefore, Blimp 1 is generally considered a "master regulator" for hematopoietic stem cells.

Blimp1 is a nuclear protein with five zinc fingers in its carboxy terminus. The Blimp-1 protein is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. BLIMP-1 has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

Long Name

B Lymphocyte Induced Maturation Protein

Alternate Names

PRDM1, ZNFPR1A1

Entrez Gene IDs

639 (Human); 12142 (Mouse); 309871 (Rat)

Gene Symbol

PRDM1

UniProt

NP_001189.2 (Human)

Additional BLIMP1/PRDM1 Products

Product Documents for BLIMP1/PRDM1 Antibody

Certificate of Analysis

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Product Specific Notices for BLIMP1/PRDM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BLIMP1/PRDM1 Antibody

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Protocols

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