BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80599

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # LLBmi1-1

Format

Azide and BSA Free
View all BMI-1 Primary Antibodies »

Product Specifications

Immunogen

Human Bmi1 protein [Swiss-Prot# P35226]

Localization

Nucleus. Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free

Western Blot: BMI-1 Antibody (LLBmi1-1)Azide and BSA Free [NBP2-80599]

Western Blot: BMI-1 Antibody (LLBmi1-1)Azide and BSA Free [NBP2-80599]

Western Blot: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using NBP1-96140. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599]

Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599]

Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-BMI-1 Antibody (LLBmi1-1) NBP2-80599 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: BMI-1 Antibody (LLBmi1-1)Azide and BSA Free [NBP2-80599]

Flow Cytometry: BMI-1 Antibody (LLBmi1-1)Azide and BSA Free [NBP2-80599]

Flow Cytometry: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.
Western Blot: BMI-1 Antibody (LLBmi1-1)Azide and BSA Free [NBP2-80599]

Western Blot: BMI-1 Antibody (LLBmi1-1)Azide and BSA Free [NBP2-80599]

Western Blot: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599]

Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599]

Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-BMI-1 Antibody (LLBmi1-1) NBP2-80599 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Flow Cytometry: BMI-1 Antibody (LLBmi1-1)Azide and BSA Free [NBP2-80599]

Flow Cytometry: BMI-1 Antibody (LLBmi1-1)Azide and BSA Free [NBP2-80599]

Flow Cytometry: BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free [NBP2-80599] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher). Image from the standard format of this antibody.

Applications for BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
This Bmi1 Antibody (LLBmi1-1) is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation and Western blot, where a band can be seen at approximately 42 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMI-1

Bmi1 is a member of the polycomb group (PcG) multiprotein PRC1 complex which functions to control axial skeleton development through Hox gene repression. Bmi1 plays an important role in repressing the transcription of the p16Ink4a and p19Arf genes and is required to maintain the proliferative and self-renewal properties of adult hematopoietic and neural stem cells. Bmi1 is an oncogene whose overexpression has been associated with many types of human tumors, including those in breast, colorectal, prostate, and non-small cell lung cancers. As seen in many recent publications, Bmi1 antibodies can serve as useful research tools to study Bmi1 and its effect on tumor proliferation and tumorigenesis.

Long Name

B Lymphoma Mo-MLV Insertion Region 1

Alternate Names

BMI1, PCGF4, RNF51

Gene Symbol

BMI1

Additional BMI-1 Products

Product Documents for BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for BMI-1 Antibody (LLBmi1-1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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