BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free

Novus Biologicals | Catalog # NBP2-37421

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4B7B2

Format

BSA Free
View all BMPR-IA/ALK-3 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human BMPR-IA/ALK-3 (AA: 179-378) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free

Western Blot: BMPR-IA/ALK-3 Antibody (4B7B2)BSA Free [NBP2-37421]

Western Blot: BMPR-IA/ALK-3 Antibody (4B7B2)BSA Free [NBP2-37421]

Western Blot: BMPR-IA/ALK-3 Antibody (4B7B2) [NBP2-37421] - Analysis using BMPR1A mAb against HEK293 (1) and BMPR1A (AA: 179-378)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

Immunohistochemistry-Paraffin: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

Immunohistochemistry-Paraffin: BMPR-IA/ALK-3 Antibody (4B7B2) [NBP2-37421] - Analysis of kidney tissues using BMPR1A mouse mAb with DAB staining.
Flow Cytometry: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

Flow Cytometry: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

Flow Cytometry: BMPR-IA/ALK-3 Antibody (4B7B2) [NBP2-37421] - An intracellular stain was performed on Jurkat cells with BMPR-IA/ALK-3 antibody (4B7B2) NBP2-37421PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Western Blot: BMPR-IA/ALK-3 Antibody (4B7B2)BSA Free [NBP2-37421]

Western Blot: BMPR-IA/ALK-3 Antibody (4B7B2)BSA Free [NBP2-37421]

Western Blot: BMPR-IA/ALK-3 Antibody (4B7B2) [NBP2-37421] - Analysis using BMPR1A mAb against human BMPR1A recombinant protein. (Expected MW is 48.1 kDa)
Immunohistochemistry-Paraffin: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

Immunohistochemistry-Paraffin: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

Immunohistochemistry-Paraffin: BMPR-IA/ALK-3 Antibody (4B7B2) [NBP2-37421] - Analysis of colon cancer tissues using BMPR1A mouse mAb with DAB staining.
Flow Cytometry: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

Flow Cytometry: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

Flow Cytometry: BMPR-IA/ALK-3 Antibody (4B7B2) [NBP2-37421] - Analysis of Jurkat cells using BMPR1A mouse mAb (green) and negative control (purple).
ELISA: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

ELISA: BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free [NBP2-37421]

ELISA: BMPR-IA/ALK-3 Antibody (4B7B2) [NBP2-37421] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 26728496). This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMPR-IA/ALK-3

The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding.

Long Name

Bone Morphogenetic Protein Receptor IA/Activin Receptor-like Kinase 3

Alternate Names

ALK-3, BMPR1A, BMPRIA, CD292

Gene Symbol

BMPR1A

Additional BMPR-IA/ALK-3 Products

Product Documents for BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free

Certificate of Analysis

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Product Specific Notices for BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BMPR-IA/ALK-3 Antibody (4B7B2) - BSA Free

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Protocols

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