BMPR-II Antibody

Novus Biologicals | Catalog # NBP1-32218

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Goat

Predicted:

Bovine (93%), Mouse (92%), Porcine (92%), Rat (92%), Rhesus Macaque (98%), Sheep (93%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all BMPR-II Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BMPR-II. The exact sequence is proprietary.

Reactivity Notes

Chicken (86%). Goat reactivity reported from a verified customer review.

Localization

Membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BMPR-II Antibody

Western Blot: BMPR-II Antibody [NBP1-32218]

Western Blot: BMPR-II Antibody [NBP1-32218]

Western Blot: BMPR-II Antibody [NBP1-32218] - Wild-type (WT) and BMPR2 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BMPR2 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: BMPR-II Antibody [NBP1-32218]

Western Blot: BMPR-II Antibody [NBP1-32218]

Western Blot: BMPR-II Antibody [NBP1-32218] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BMPR2 antibody [C1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
BMPR-II Antibody

Western Blot: Rabbit Polyclonal BMPR-II Antibody [NBP1-32218]

Western Blot: Rabbit Polyclonal BMPR-II Antibody [NBP1-32218] - Bone lysate homogenate from goats. SDS-PAGE with 0.3 mg of total protein from the homogenate per well. BMP-2, NBP1-32218, was used at a dilution of 1:1000 and RUNX/CBFA1, NBP2-24755 was used at a dilution of 1:1000 in TBS Tween and 5% powdered milk. The protein was labeled at 150 kDa. Quantification of BMP-2 in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. Evaluation of pre-cellularized graft in the iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to the tibial bone defect, G2: cellularized graft for 6 weeks applied to the tibial bone defect, G3: cellularized graft for 8 weeks applied to the tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 120 days after graft implantation. Image from a verified customer review.
BMPR-II Antibody

Immunohistochemistry-Paraffin: Rabbit Polyclonal BMPR-II Antibody [NBP1-32218]

The sections of histological sections of goat bone fragments were deparaffinized, peroxidase blocked in methanol and 30% hydrogen peroxide (9:1), and antigen retrieval was performed with porcine gastric pepsin and HCl at pH 3.0 in an oven at 37°C for 30 minutes. Then, blocking with BSA was performed for 1 hour followed by incubation with the primary antibody BMPR-2 antibody NBP1-32218 (1:100) overnight. Subsequently, excess primary antibody was removed with PBS pH 7.6 and incubated with the secondary antibody (goat anti-rabbit IgG - NB7160 (1:200) for 1 hour and polymer was applied for 30 minutes. Finally, 150 μL of DAB was applied for five minutes and counterstained with hematoxylin. Image from a verified customer review.

Applications for BMPR-II Antibody

Application
Recommended Usage

Immunohistochemistry

Validated for IHC from a verified customer review.

Immunohistochemistry-Paraffin

Validated for IHC-P from a verified customer review.

Western Blot

1:500-1:3000

Reviewed Applications

Read 2 reviews rated 5 using NBP1-32218 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BMPR-II

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease. [provided by RefSeq]

Long Name

Bone Morphogenetic Protein Receptor II

Alternate Names

BMPR2, BMPRII

Gene Symbol

BMPR2

Additional BMPR-II Products

Product Documents for BMPR-II Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BMPR-II Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

BMP Family
Mesenchymal Stem Cell Markers

Customer Reviews for BMPR-II Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • BMPR-II Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Bone and Bone Extracts
    Species: Goat
    Verified Customer | Posted 07/29/2025
    The f histological goat bone fragments were incubated with the primary antibody BMPR22 NBP1-32218 overnight. Subsequently, the secondary antibody goat anti-rabbit IgG - NB7160 was applied and incubated for 1 hour.
    Validation of an immunohistochemistry technique for goats
    BMPR-II Antibody NBP1-32218
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • BMPR-II Antibody
    Name: Inácio Viana
    Application: Western Blot
    Sample Tested: Bone and Bone Extracts
    Species: Goat
    Verified Customer | Posted 05/13/2025
    Bone lysate homogenate from goats. SDS-PAGE with 0.3 mg of total protein from the homogenate per well. BMP-2, NBP1-32218, was used at a dilution of 1:1000 in TBS Tween and 5% powdered milk. The protein was labeled at 150 kDa.
    Quantification of BMP-2 in bone graft in the tibia of intrailiac cellularized goats at different times compared with tibial autograft and untreated bone defect. Evaluation of pre-cellularized graft in the iliac crest in the treatment of bone defects in the tibia of goats. G1: cellularized graft for 4 weeks applied to the tibial bone defect, G2: cellularized graft for 6 weeks applied to the tibial bone defect, G3: cellularized graft for 8 weeks applied to the tibial bone defect, G4: tibial autograft and G5: untreated bone defect. All groups were evaluated 120 days after graft implantation.
    BMPR-II Antibody NBP1-32218
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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