BOP-JF549
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-(((1-((3R,5S)-5-(((S)-1-carboxy-2-(4-((pyrrolidine-1-carbonyl)oxy)phenyl)ethyl)carbamoyl)-1-(phenylsulfonyl)pyrrolidin-3-yl)-1H-1,2,3-triazol-4-yl)methyl)carbamoyl)benzoate
Purity: ≥85%
Biological Activity
BOP-JF549 is a fluorescent dual α9β1/α4β1 integrin inhibitor. Comprises BOP (Cat. No. 6047) conjugated to Janelia Fluor® 549 (Cat. No. 6147). Fluorogenic: fluoresces only once bound to integrins, enabling hassle-free no-wash experiments. Bright and photostable, enabling live cell tracking of integrin receptors over long time-course experiments. Excitation maximum = 549 nm; emission maximum = 571 nm.
Application of BOP-JF549 in Immortalized Human Keratinocyte Cells
Immortalized human keratinocytes expressing a GFP-tagged α4 integrin were incubated with 10 μm BOP-Janelia Fluor® 568. Cells were incubated at 37°C and imaged using Nikon A1R confocal microscope. Scale bar = 10 μm.
Image kindly provided by Willow Hight-Warburton, Kings College London.
Optical Data for BOP-JF549
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Closest Laser line | 561 nm |
| Application | Flow Cytometry, Confocal Microscopy, super resolution microscopy (SRM) |
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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