BOP-JF549

Catalog # Availability Size / Price Qty
6996/100U
BOP-JF549 | Fluorescent Integrins Probes
1 Image
Description: Yellow fluorescent dual α9β1/α4β1 integrin inhibitor comprising BOP conjugated to Janelia Fluor® 549; fluorogenic; photostable

Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-(((1-((3R,5S)-5-(((S)-1-carboxy-2-(4-((pyrrolidine-1-carbonyl)oxy)phenyl)ethyl)carbamoyl)-1-(phenylsulfonyl)pyrrolidin-3-yl)-1H-1,2,3-triazol-4-yl)methyl)carbamoyl)benzoate

Purity: ≥85%

Product Details
Reviews

Biological Activity

BOP-JF549 is a fluorescent dual α9β1/α4β1 integrin inhibitor. Comprises BOP (Cat. No. 6047) conjugated to Janelia Fluor® 549 (Cat. No. 6147). Fluorogenic: fluoresces only once bound to integrins, enabling hassle-free no-wash experiments. Bright and photostable, enabling live cell tracking of integrin receptors over long time-course experiments. Excitation maximum = 549 nm; emission maximum = 571 nm.
Application of BOP-JF549

Application of BOP-JF549 in Immortalized Human Keratinocyte Cells

Immortalized human keratinocytes expressing a GFP-tagged α4 integrin were incubated with 10 μm BOP-Janelia Fluor® 568. Cells were incubated at 37°C and imaged using Nikon A1R confocal microscope. Scale bar = 10 μm.

Image kindly provided by Willow Hight-Warburton, Kings College London.

Optical Data for BOP-JF549

BOP-JF549 Dye Spectra
Emission Color Yellow
Brightness
λabs 549 nm
λem 571 nm
Closest Laser line 561 nm
Application Flow Cytometry, Confocal Microscopy, super resolution microscopy (SRM)

Technical Data

M.Wt:
1048.14
Formula:
C55H53N9O11S
Purity:
≥85%
Storage:
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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