BRD4 Antibody (CL1115)

Novus Biologicals | Catalog # NBP3-52064

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL1115
View all BRD4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O60885, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for BRD4 Antibody (CL1115)

BRD4 Antibody (CL1115) Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (CL1115) [NBP3-52064]

Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (CL1115) [NBP3-52064]

Staining of MCF-7 cells showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
BRD4 Antibody (CL1115) Immunohistochemistry: BRD4 Antibody (CL1115) [NBP3-52064]

Immunohistochemistry: BRD4 Antibody (CL1115) [NBP3-52064]

Staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
BRD4 Antibody (CL1115) Immunohistochemistry: BRD4 Antibody (CL1115) [NBP3-52064]

Immunohistochemistry: BRD4 Antibody (CL1115) [NBP3-52064]

Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
BRD4 Antibody (CL1115) Western Blot: BRD4 Antibody (CL1115) [NBP3-52064]

Western Blot: BRD4 Antibody (CL1115) [NBP3-52064]

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
BRD4 Antibody (CL1115) Immunohistochemistry: BRD4 Antibody (CL1115) [NBP3-52064]

Immunohistochemistry: BRD4 Antibody (CL1115) [NBP3-52064]

Staining of human stomach cancer shows moderate nuclear positivity in tumor cells.
BRD4 Antibody (CL1115) Immunohistochemistry: BRD4 Antibody (CL1115) [NBP3-52064]

Immunohistochemistry: BRD4 Antibody (CL1115) [NBP3-52064]

Staining of human kidney shows moderate nuclear positivity in cells in renal glomeruli and in tubules.

Applications for BRD4 Antibody (CL1115)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRD4

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 Antibody (CL1115)

Certificate of Analysis

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Product Specific Notices for BRD4 Antibody (CL1115)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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