Brg1 Antibody (CL13783) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43830

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13783

Format

Azide and BSA Free
View all Brg1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P51532, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Brg1 Antibody (CL13783) - Azide and BSA Free

Brg1 Antibody (CL13783) - Azide and BSA Free Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Analysis in human testis and liver tissues using NBP3-43830 antibody. Corresponding Brg1 RNA-seq data are presented for the same tissues.
Brg1 Antibody (CL13783) - Azide and BSA Free Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Staining of human endometrial cancer shows strong nuclear positivity in tumor cells.
Brg1 Antibody (CL13783) - Azide and BSA Free Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Staining of human liver shows no positivity in hepatocytes as expected.
Brg1 Antibody (CL13783) - Azide and BSA Free Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.
Brg1 Antibody (CL13783) - Azide and BSA Free Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.
Brg1 Antibody (CL13783) - Azide and BSA Free Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Brg1 Antibody (CL13783) - Azide and BSA Free Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Staining of human small intestine shows strong nuclear positivity in glandular cells.
Brg1 Antibody (CL13783) - Azide and BSA Free Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.
Brg1 Antibody (CL13783) - Azide and BSA Free Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Immunohistochemistry-Paraffin: Brg1 Antibody [NBP3-43830]

Staining of human testis cancer (seminoma) shows strong nuclear positivity in tumor cells.

Applications for Brg1 Antibody (CL13783) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody (CL13783) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Brg1 Antibody (CL13783) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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