Brg1 Antibody (SN20-03)

Novus Biologicals | Catalog # NBP2-74964

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN20-03 expressed in HEK293
View all Brg1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human Brg1 aa 240-280. (SwissProt: P51532 Human; SwissProt: Q3TKT4 Mouse; SwissProt: Q8K1P7 Rat)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Brg1 Antibody (SN20-03)

Western Blot: Brg1 Antibody (SN20-03) [NBP2-74964]

Western Blot: Brg1 Antibody (SN20-03) [NBP2-74964]

Western Blot: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of BRG1 on different lysates using anti-BRG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: HepG2
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded mouse colon tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunocytochemistry/Immunofluorescence: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Staining BRG1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded human kidney tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded human tonsil tissue using anti-BRG1 antibody. Counter stained with hematoxylin.
Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) [NBP2-74964]

Immunohistochemistry: Brg1 Antibody (SN20-03) - BSA Free [NBP2-74964] - Analysis of paraffin-embedded mouse testis tissue using anti-BRG1 antibody. Counter stained with hematoxylin.

Applications for Brg1 Antibody (SN20-03)

Application
Recommended Usage

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:50-1:1000

Western Blot

1:1000-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brg1

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Long Name

BRM/SWI2-related gene 1

Alternate Names

SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2

Gene Symbol

SMARCA4

Additional Brg1 Products

Product Documents for Brg1 Antibody (SN20-03)

Certificate of Analysis

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Product Specific Notices for Brg1 Antibody (SN20-03)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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