BTBD7 Antibody

Novus Biologicals | Catalog # NBP1-49652

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all BTBD7 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 1050-1150 on human BTBD7 protein.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 11231300)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BTBD7 Antibody

Western Blot: BTBD7 Antibody [NBP1-49652]

Western Blot: BTBD7 Antibody [NBP1-49652]

Western Blot: BTBD7 Antibody [NBP1-49652] - Human 293T cells were infected by lentivirus overexpression of control or BTBD7 for 72h. Total cell lysates were subjected to Western blot. PVDF membrane was probed with BTBD7 Antibody. A specific band was detected for BTBD7 at approximately 138 kDa. Image from verified customer review.
Western Blot: BTBD7 Antibody [NBP1-49652]

Western Blot: BTBD7 Antibody [NBP1-49652]

Western Blot: BTBD7 Antibody [NBP1-49652] - Lane A: Immune Serum, Lane B: Preimmune. Apparent MW is 138 kDa

Applications for BTBD7 Antibody

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:200

Western Blot

1:5000
Application Notes
Use in IHC-Paraffin reported in scientific literature (PMID: 23325674).

Reviewed Applications

Read 1 review rated 4 using NBP1-49652 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BTBD7

Cancer cells especially human hepatocellular carcinoma (HCC) differentially express many genes as determined by cDNA microarrays. One of these genes is Btbd7 whose function was not known. When transfected in NIH3T3 cells this gene exhibited enhanced cell proliferation activity by MTT assay. The enhanced tumorigencity was blocked by anti-sense RNA experiments (1). Recently it has been shown that Btbd7 regulate epithelial cell dynamics and branching morphogenesis. The organ development during embryogenesis requires extensive branching of epithelia via formation of cleft and buds. These branches are developed by local outgrowth or by formation of cleft that further divide epithelia in to buds. The bud or tubule extension is controlled and regulated by various growth factors. These branching finally led to formation of various organs. Cellular matrix protein fibronectin is required for salivary, lung and kidney branching (3). The edges of fibronectin translocates inwards as cleft accompanied by loss of E-cadherin in cells adjacent to fibronectin. The Btbd7 activity was noticed at cell cleft forming sites (2). The cleft formation involves buds delineation of buds by conversion of epithelial cell-cell adhesion to cell-matrix adhesion. Btbd7 provides a mechanistic link between the extracellular matrix and cleft propagation via its highly discrete expression that control epithelial cell motility by interaction with other cellular architectural and scaffolding proteins like Snail2 (slug), E-cadherin (4). The Btbd7 antibodies were generated using peptide corresponding to C-terminal domain of the human Btbd7 gene. The Btbd7 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer. Antibodies to Btbd7 will label ~144kDa protein in Western blot positive control for Btbd7.

Alternate Names

BTB (POZ) domain containing 7, DKFZp686N0544, FLJ10648, FUP1BTB/POZ domain-containing protein 7, KIAA1525, MGC48310

Gene Symbol

BTBD7

UniProt

NP_001002860 (Human)

Additional BTBD7 Products

Product Documents for BTBD7 Antibody

Certificate of Analysis

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Product Specific Notices for BTBD7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BTBD7 Antibody (1)

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  • BTBD7 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T cell line
    Species: Human
    Verified Customer | Posted 07/17/2018
    293T cells were infected by lentivirus overexpression of control or BTBD7 for 72h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1mm/ml Human BTBD7 Antibody. A specific band was detected for BTBD7 at approximately 138 kDa. This experiment was conducted under reducing conditions
    BTBD7 Antibody NBP1-49652

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