BTK [p Tyr223] Antibody

Novus Biologicals | Catalog # NBP3-12916

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all BTK Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr223 of human BTK. The exact sequence is proprietary.

Modification

p Tyr223

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for BTK [p Tyr223] Antibody

Western Blot: BTK [p Tyr223] Antibody [NBP3-12916]

Western Blot: BTK [p Tyr223] Antibody [NBP3-12916]

Western Blot: BTK [p Tyr223] Antibody [NBP3-12916] - Untreated (-) and treated (+) Raji whole cell extracts (1 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:6000.
Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916]

Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916]

Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Rat lymph node. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916]

Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916]

Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Mouse spleen. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916]

Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916]

Immunohistochemistry-Paraffin: BTK [p Tyr223] Antibody [NBP3-12916] - Mouse thymus gland. BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (NBP3-12916) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for BTK [p Tyr223] Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BTK

Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. All members of the BTK/Tec family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Long Name

Bruton Agammaglobulinemia Tyrosine Kinase

Alternate Names

AGMX1, IMD1, PSCTK1, XLA

Gene Symbol

BTK

Additional BTK Products

Product Documents for BTK [p Tyr223] Antibody

Certificate of Analysis

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Product Specific Notices for BTK [p Tyr223] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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