BYSL Antibody - BSA Free

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89501]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89501] - Staining of human testis shows moderate positivity in nucleoli in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89501]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89501] - Staining of human lymph node shows strong positivity in nucleoli in lymphoid cells.

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89501]

Immunohistochemistry-Paraffin: BYSL Antibody [NBP1-89501] - Staining of human rectum shows strong positivity in nucleoli in glandular cells.

Western Blot: BYSL Antibody [NBP1-89501]

Western Blot: BYSL Antibody [NBP1-89501] - Analysis using Anti-BYSL antibody NBP1-89501 (A) shows similar pattern to independent antibody NBP1-89500 (B).

Western Blot: BYSL Antibody - BSA Free [NBP1-89501]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Western Blot: BYSL Antibody - BSA Free [NBP1-89501] -

BYSL is related to the poor prognosis of patients with osteosarcoma. (A) Cluster heatmap of differentially expressed genes in GSE126209. (B) Overall survival was compared between osteosarcoma patients with high and low BYSL expression in TCGA database. (C) Immunocytochemical staining of BYSL in osteosarcoma tissues (n = 51, scale bar = 50 um). (D) Overall survival was compared between osteosarcoma patients with high and low BYSL expression in an in-house cohort. (E) MG63 and Saos-2 cells were transfected with the control plasmid (oe-NC) or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic or normoxic conditions. After nuclear and cytosolic separation, protein levels of Nrf2, BYSL, Histone H3, and beta -Actin were measured by western blot. (F) MG63 and Saos-2 cells were transfected with control plasmid (oe-NC), BYSL overexpression plasmid (oe-BYSL), si-control (si-NC), or si-Nrf2, and then cultured under hypoxic condtions. The protein levels of Nrf2, E-cadherin, N-cadheirn, Vimentin, and beta -Actin were measured by western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35154253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BYSL Antibody - BSA Free [NBP1-89501] -

BYSL knockdown partially abolishes miR-378a-3p-mediated osteosarcoma cell epithelial-to-mesenchymal transition (EMT), invasion, migration, and apoptosis under normoxia. (A) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC) or miR-378a-3p-inhibitor, and then cultured under normoxic conditions. The RNA level of miR-378a-3p was measured by RT-qPCR. (B,C) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC), miR-378a-3p-inhibitor, si-control (si-NC), or si-BYSL, and then cultured under normoxic conditions. The protein levels of Bax, Bcl-2, E-cadherin, N-cadherin, vimentin, BYSL and Nrf2 were measured by western blot. (D) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC), miR-378a-3p-inhibitor, si-control (si-NC), or si-BYSL, and then cultured under normoxic conditions. Cell apoptosis was measured by flow cytometry. (E) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC), miR-378a-3p-inhibitor, si-control (si-NC), or si-BYSL, and then cultured under normoxic conditions. Cell invasion was measured by matrigel invasion assay. Scale bar = 100 um. (F) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC), miR-378a-3p-inhibitor, si-control (si-NC), or si-BYSL, and then cultured under normoxic conditions. Cell migration was measured by scratch wound healing assay. Scale bar = 500 um. The data are presented as the mean +/- SD. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35154253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BYSL Antibody - BSA Free [NBP1-89501] -

BYSL is a direct target of miR-378a-3p. (A) MG63 and Saos-2 cells were cultured under hypoxic or normoxic conditions. The RNA levels of miR-378a-3p and BYSL were measured by RT-qPCR. (B) The 3′-untranslated region (UTR) of BYSL harbor potential miR-378a-3p binding sites. (C) The luciferase activity displayed by the luciferase reporter constructs which contained wild-type (WT) or mutant (MUT) 3′-UTR of BYSL were co-transfected with miR-378a-3p mimic into MG63 and Saos-2 cells. (D) MG63 and Saos-2 cells were transfected with control-mimic (miR-378a-3p-NC) or miR-378a-3p-mimic, and then cultured under hypoxic or normoxic conditions. The protein levels of BYSL, E-cadherin, N-cadherin, and Vimentin were measured by western blot. The data are presented as the mean +/- SD. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35154253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BYSL Antibody - BSA Free [NBP1-89501] -

BYSL knockdown partially abolishes miR-378a-3p-mediated osteosarcoma cell epithelial-to-mesenchymal transition (EMT), invasion, migration, and apoptosis under normoxia. (A) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC) or miR-378a-3p-inhibitor, and then cultured under normoxic conditions. The RNA level of miR-378a-3p was measured by RT-qPCR. (B,C) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC), miR-378a-3p-inhibitor, si-control (si-NC), or si-BYSL, and then cultured under normoxic conditions. The protein levels of Bax, Bcl-2, E-cadherin, N-cadherin, vimentin, BYSL and Nrf2 were measured by western blot. (D) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC), miR-378a-3p-inhibitor, si-control (si-NC), or si-BYSL, and then cultured under normoxic conditions. Cell apoptosis was measured by flow cytometry. (E) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC), miR-378a-3p-inhibitor, si-control (si-NC), or si-BYSL, and then cultured under normoxic conditions. Cell invasion was measured by matrigel invasion assay. Scale bar = 100 um. (F) MG63 and Saos-2 cells were transfected with control-inhibitor (miR-NC), miR-378a-3p-inhibitor, si-control (si-NC), or si-BYSL, and then cultured under normoxic conditions. Cell migration was measured by scratch wound healing assay. Scale bar = 500 um. The data are presented as the mean +/- SD. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35154253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BYSL Antibody - BSA Free [NBP1-89501] -

BYSL overexpression rescues the effect of miR-378a-3p overexpression on osteosarcoma cells under hypoxia. (A) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC) or miR-378a-3p-mimic, and then cultured under hypoxic conditions. The RNA level of miR-378a-3p was measured by RT-qPCR. (B,C) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC), miR-378a-3p-mimic, control plasmid (oe-NC), or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic conditions. The protein levels of Bax, Bcl-2, E-cadherin, N-cadherin, vimentin, BYSL and Nrf2 were measured by western blot. (D) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC), miR-378a-3p-mimic, control plasmid (oe-NC), or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic conditions. Cell apoptosis was measured by flow cytometry. (E) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC), miR-378a-3p-mimic, control plasmid (oe-NC), or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic conditions. Cell invasion was measured by matrigel invasion assay. Scale bar = 100 um. (F) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC), miR-378a-3p-mimic, control plasmid (oe-NC), or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic conditions. Cell migration was measured by scratch wound healing assay. Scale bar = 500 um. The data are presented as the mean +/- SD. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35154253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BYSL Antibody - BSA Free [NBP1-89501] -

BYSL overexpression rescues the effect of miR-378a-3p overexpression on osteosarcoma cells under hypoxia. (A) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC) or miR-378a-3p-mimic, and then cultured under hypoxic conditions. The RNA level of miR-378a-3p was measured by RT-qPCR. (B,C) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC), miR-378a-3p-mimic, control plasmid (oe-NC), or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic conditions. The protein levels of Bax, Bcl-2, E-cadherin, N-cadherin, vimentin, BYSL and Nrf2 were measured by western blot. (D) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC), miR-378a-3p-mimic, control plasmid (oe-NC), or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic conditions. Cell apoptosis was measured by flow cytometry. (E) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC), miR-378a-3p-mimic, control plasmid (oe-NC), or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic conditions. Cell invasion was measured by matrigel invasion assay. Scale bar = 100 um. (F) MG63 and Saos-2 cells were transfected with control-mimic (miR-NC), miR-378a-3p-mimic, control plasmid (oe-NC), or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic conditions. Cell migration was measured by scratch wound healing assay. Scale bar = 500 um. The data are presented as the mean +/- SD. *p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35154253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BYSL Antibody - BSA Free [NBP1-89501] -

BYSL is related to the poor prognosis of patients with osteosarcoma. (A) Cluster heatmap of differentially expressed genes in GSE126209. (B) Overall survival was compared between osteosarcoma patients with high and low BYSL expression in TCGA database. (C) Immunocytochemical staining of BYSL in osteosarcoma tissues (n = 51, scale bar = 50 um). (D) Overall survival was compared between osteosarcoma patients with high and low BYSL expression in an in-house cohort. (E) MG63 and Saos-2 cells were transfected with the control plasmid (oe-NC) or BYSL overexpression plasmid (oe-BYSL), and then cultured under hypoxic or normoxic conditions. After nuclear and cytosolic separation, protein levels of Nrf2, BYSL, Histone H3, and beta -Actin were measured by western blot. (F) MG63 and Saos-2 cells were transfected with control plasmid (oe-NC), BYSL overexpression plasmid (oe-BYSL), si-control (si-NC), or si-Nrf2, and then cultured under hypoxic condtions. The protein levels of Nrf2, E-cadherin, N-cadheirn, Vimentin, and beta -Actin were measured by western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35154253), licensed under a CC-BY license. Not internally tested by Novus Biologicals.