c-jun Antibody (CL9662) - Azide and BSA Free

Name: c-jun Antibody (CL9662) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-44268
Price: 579 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-44268

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL9662

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P05412, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for c-jun Antibody (CL9662) - Azide and BSA Free

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Staining of human endometrium shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Staining of human prostate shows moderate nuclear positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence: c-jun Antibody [NBP3-44268]

Staining of U-251 MG cells using the Anti-c-jun monoclonal antibody).

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-44268]

Staining of human thyroid gland shows moderate nuclear positivity in glandular cells.

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Applications for c-jun Antibody (CL9662) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200

Application Notes

Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: c-Jun

The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Alternate Names

cJun, JUN

Gene Symbol

JUN

Additional c-Jun Products

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Product Specific Notices for c-jun Antibody (CL9662) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.