c-jun Antibody (CL9662) - BSA Free

Name: c-jun Antibody (CL9662) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-21172
Price: 519 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-21172

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL9662

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P05412, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for c-jun Antibody (CL9662) - BSA Free

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-21172] -

Staining of human thyroid gland shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-21172] -

Staining of human endometrium shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-21172] -

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: c-jun Antibody [NBP3-21172] -

Staining of human prostate shows moderate nuclear positivity in glandular cells.

Immunocytochemistry/Immunofluorescence: c-jun Antibody [NBP3-21172] -

Staining of U-251 MG cells using the Anti-JUN monoclonal antibody, showing specific staining in the nucleoplasm and aggresomes in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

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Applications for c-jun Antibody (CL9662) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Application Notes

ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: c-Jun

The human protooncogene JUN is the putative transforming gene of avian sarcoma virus 17, and it encodes a protein which is highly homologous to the viral protein. cJun (previously known as the Fos binding protein p39) and c Fos form a complex in the nucleus. AP 1 (activating protein 1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF subunits that bind to a common DNA site, the AP1 binding site. AP 1 proteins, mostly the Jun group, regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16. Fos and Jun proto oncogene expression is induced transiently by a variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons. JUN has been mapped to 1p32 to p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Long Name

Cellular Repressor of E1A-stimulated Genes/Transcription Factor AP-1

Alternate Names

cJun, JUN

Gene Symbol

JUN

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Product Specific Notices for c-jun Antibody (CL9662) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.