c-Myc Antibody (9E11) - Azide and BSA Free

Name: c-Myc Antibody (9E11) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-80674
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-80674

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken, Yeast

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 9E11

Format

Azide and BSA Free

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Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 408-420 (AEEQKLISEEDL) of human c-Myc Antibody (9E11), conjugated to KLH. [UniProt# P01106]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 15777849).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse c-Myc Antibody (9E11) - Azide and BSA Free (NB200-108) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for c-Myc Antibody (9E11) - Azide and BSA Free

Western Blot: c-Myc Antibody (9E11)Azide and BSA Free [NBP2-80674]

Western Blot: c-Myc Antibody (9E11) - Azide and BSA Free [NBP2-80674] - Whole cell protein from PC3 (lane 1), U-2 OS (lane 2) and mouse testis (lane 3) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-c-Myc in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.

Immunohistochemistry: c-Myc Antibody (9E11) - Azide and BSA Free [NBP2-80674]

Immunohistochemistry: c-Myc Antibody (9E11) - Azide and BSA Free [NBP2-80674] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB200-108, clone 9E11) at 1:600 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP po

Flow Cytometry: c-Myc Antibody (9E11) - Azide and BSA Free [NBP2-80674]

Flow Cytometry: c-Myc Antibody (9E11) - Azide and BSA Free [NBP2-80674] - An intracellular stain was performed on U-937 cells with c-Myc Antibody [9E11] NB200-108 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody. Image from the standard format of this antibody.

Flow Cytometry: c-Myc Antibody (9E11) - Azide and BSA Free [NBP2-80674]

Flow Cytometry: c-Myc Antibody (9E11) - Azide and BSA Free [NBP2-80674] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E11) NB200-108PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerthrin.

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Applications for c-Myc Antibody (9E11) - Azide and BSA Free

Application

Recommended Usage

Chromatin Immunoprecipitation (ChIP)

2 ug

ELISA

1:100-1:2000

Flow Cytometry

1:200-1:400

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

2ug/mg lysate

Western Blot

1:500-1:1000

Application Notes

This c-Myc antibody (clone 9E11) is useful for Flow Cytometry, ChIP, Immunoprecipitation, ELISA, Immunohistochemistry- Frozen and Paraffin and Western blot. This antibody is CyTOF ready.

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Advanced Features

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: c-Myc

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N.,... Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

Long Name

v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)

Alternate Names

cMyc, Myc, Myc2, Niard, Nird

Gene Symbol

MYC

Additional c-Myc Products

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Product Specific Notices for c-Myc Antibody (9E11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.