c-Myc Antibody (Myc.A7) - Azide and BSA Free

Name: c-Myc Antibody (Myc.A7) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-37822
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-37822

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # Myc.A7

Format

Azide and BSA Free

View all c-Myc Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide: EQKLISEEDL c-Myc Antibody (Myc.A7).

Specificity

Detects N-terminal or C-terminal Myc tagged fusion proteins. It has been successfully used in ELISA, immunofluorescence, immunoprecipitation and Western Blot procedures. immunogen is a synthetic peptide EQKLISEEDL (Myc).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for c-Myc Antibody (Myc.A7) - Azide and BSA Free

Western Blot: c-Myc Antibody (Myc.A7)Azide and BSA Free [NBP2-37822]

Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.

Immunocytochemistry/ Immunofluorescence: c-Myc Antibody (Myc.A7) - Azide and BSA Free [NBP2-37822]

Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.

Flow Cytometry: c-Myc Antibody (Myc.A7) - Azide and BSA Free [NBP2-37822]

Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: c-Myc Antibody (Myc.A7)Azide and BSA Free [NBP2-37822]

Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)

Western Blot: c-Myc Antibody (Myc.A7)Azide and BSA Free [NBP2-37822]

Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.

Immunocytochemistry/ Immunofluorescence: c-Myc Antibody (Myc.A7) - Azide and BSA Free [NBP2-37822]

Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 488 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.

Immunocytochemistry/ Immunofluorescence: c-Myc Antibody (Myc.A7) - Azide and BSA Free [NBP2-37822]

Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 550 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at

Flow Cytometry: c-Myc Antibody (Myc.A7) - Azide and BSA Free [NBP2-37822]

Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on HepG2 cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

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Applications for c-Myc Antibody (Myc.A7) - Azide and BSA Free

Application

Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:500 - 1:2000

Immunoprecipitation

1:10 -1:500

Western Blot

1:1000 - 1:3000

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: c-Myc

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N.,... Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

Long Name

v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)

Alternate Names

cMyc, Myc, Myc2, Niard, Nird

Gene Symbol

MYC

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Product Specific Notices for c-Myc Antibody (Myc.A7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.