C9orf72 Antibody (779) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13507

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Xenopus (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 779

Format

Azide and BSA Free
View all C9orf72 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for C9orf72 Antibody (779) - Azide and BSA Free

Western Blot: C9orf72 Antibody (779) [NBP3-13507]

Western Blot: C9orf72 Antibody (779) [NBP3-13507]

Western Blot: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 Antibody (GT779) [NBP3-13507] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse brain. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: C9orf72 Antibody (779) [NBP3-13507]

Western Blot: C9orf72 Antibody (779) [NBP3-13507]

Western Blot: C9orf72 Antibody (GT779) [NBP3-13507] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody (NBP3-13507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Western Blot: C9orf72 Antibody (779) [NBP3-13507]

Western Blot: C9orf72 Antibody (779) [NBP3-13507]

Western Blot: C9orf72 Antibody (GT779) [NBP3-13507] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (NBP3-13507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (GT779) [NBP3-13507] - Human colon cancer. C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse hippocampus. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse hippocampus. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse hippocampus. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse brain. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507]

Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse brain. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunoprecipitation: C9orf72 Antibody (779) [NBP3-13507]

Immunoprecipitation: C9orf72 Antibody (779) [NBP3-13507]

Immunoprecipitation: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 Antibody (GT779) [NBP3-13507] - Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 ug of C9orf72 antibody [779] (NBP3-13507). Western blot analysis was performed using C9orf72 antibody [779] (NBP3-13507). EasyBlot anti-Mouse IgG was used as a secondary reagent.
C9orf72 Antibody (779)

Immunocytochemistry/ Immunofluorescence: C9orf72 Antibody (779) [NBP3-13507] -

Immunocytochemistry/ Immunofluorescence: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9orf72 stained by C9orf72 antibody [GT779] (NBP3-13507) diluted at 1:500.
Red: Tau, a Tau marker, stained by Tau antibody diluted at 1:500.
Blue: Fluoroshield with DAPI.
C9orf72 Antibody (779)

Western Blot: C9orf72 Antibody (779) [NBP3-13507] -

Western Blot: C9orf72 Antibody (779) [NBP3-13507] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (NBP3-13507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Applications for C9orf72 Antibody (779) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: C9orf72

Long Name

Chromosome 9 Open Reading Frame 72

Alternate Names

DENNL72, FTDALS1

Gene Symbol

C9orf72

Additional C9orf72 Products

Product Documents for C9orf72 Antibody (779) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for C9orf72 Antibody (779) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

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Protocols

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FAQs

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