C9orf72 Antibody (GT779-RB) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13657
Recombinant Monoclonal Antibody
![Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657]](https://resources.rndsystems.com/images/products/C9orf72-Antibody-GT779-RB-Western-Blot-NBP3-13657-img0003.jpg "Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657]")
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat, Feline
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # GT779-RB
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.
Reactivity Notes
Cat (100%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for C9orf72 Antibody (GT779-RB) - Azide and BSA Free
Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657]
Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (NBP3-13657) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657]](https://resources.rndsystems.com/images/products/C9orf72-Antibody-GT779-RB-Western-Blot-NBP3-13657-img0001.jpg "Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657]")
Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657]
Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (NBP3-13657) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
ICC/IF-C9orf72 Antibody (GT779-RB)-NBP3-13657-
ICC/IF-C9orf72 Antibody (GT779-RB)-NBP3-13657-DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C9orf72 stained by C9orf72 antibody 779-RB] diluted at 1:250.Red: Tau, a cytoskeleton marker, stained by Tau antibody [287] diluted at 1:500.Blue: Fluoroshield with DAPI.
Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657] -
Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (NBP3-13657) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657] -
Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (NBP3-13657) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: C9orf72 Antibody (GT779-RB) [NBP3-13657] -
C9orf72 antibody [GT779-RB] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779-RB] (NBP3-13657) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: C9orf72 Antibody (GT779-RB) [NBP3-13657] -
C9orf72 antibody [GT779-RB] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779-RB] (NBP3-13657) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: C9orf72 Antibody (GT779-RB) [NBP3-13657] -
Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779-RB] (NBP3-13657) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for C9orf72 Antibody (GT779-RB) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: C9orf72
Long Name
Chromosome 9 Open Reading Frame 72
Alternate Names
DENNL72, FTDALS1
Gene Symbol
C9orf72
Additional C9orf72 Products
Product Documents for C9orf72 Antibody (GT779-RB) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for C9orf72 Antibody (GT779-RB) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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