CACNA1E Antibody (N493/22) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-52365

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # N493/22

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant protein amino acids 956-1033 (ID I-II cytoplasmic loop) of mouse CACNA1E (Uniprot#: Q61290) produced recombinantly in E.Coli.

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

257 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N493/22 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N493/22 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Scientific Data Images for CACNA1E Antibody (N493/22) - Chimeric - Azide and BSA Free

Western Blot: CACNA1E Antibody (N493/22) - Chimeric - Azide and BSA Free [NBP3-52365]

Western Blot: CACNA1E Antibody (N493/22) - Chimeric - Azide and BSA Free [NBP3-52365]

Western blotting of rat brain membrane with NBP3-52364 (the BSA Free version of this antibody) recognizing endogenous CACNA1E at ~250 kDa. This antibody was detected with anti-chicken-HRP.

Applications for CACNA1E Antibody (N493/22) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CACNA1E

Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel, and partially by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), omega-conotoxin-GVIA (omega-CTx-GVIA), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels contaning alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.; Subcellular location: Membrane; Multi-pass membrane protein.; Tissue specificity: Expressed in neuronal tissues and in kidney.

Long Name

Voltage-dependent R-type calcium channel subunit alpha-1E

Alternate Names

BII, CACH6, CACNL1A6

Gene Symbol

CACNA1E

Additional CACNA1E Products

Product Documents for CACNA1E Antibody (N493/22) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CACNA1E Antibody (N493/22) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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