CACNA2D2 Antibody (N492/39) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-52363

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # N492/39

Format

Azide and BSA Free
View all CACNA2D2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein amino acids 61-197 (extracellular domain) of mouse CACNA2D2 (Uniprot#: Q6PHS9) produced recombinantly in E.Coli.

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N492/39 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N492/39 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Scientific Data Images for CACNA2D2 Antibody (N492/39) - Chimeric - Azide and BSA Free

Immunohistochemistry-Paraffin: CACNA2D2 Antibody (N492/39) - Chimeric - Azide and BSA Free [NBP3-52363]

Immunohistochemistry-Paraffin: CACNA2D2 Antibody (N492/39) - Chimeric - Azide and BSA Free [NBP3-52363]

Representative image of formalin fixed paraffin-embedded section of rat cerebellum section stained with NBP3-52362 (the BSA Free version of this antibody) (brown) and counterstained with hematoxylin (blue). This antibody specifically stains the Purkinje cells in the cerebellum.
Western Blot: CACNA2D2 Antibody (N492/39) - Chimeric - Azide and BSA Free [NBP3-52363]

Western Blot: CACNA2D2 Antibody (N492/39) - Chimeric - Azide and BSA Free [NBP3-52363]

Western blotting of rat brain membrane with NBP3-52362 (the BSA Free version of this antibody), recognizing endogenous CCACNA2D2 at ~130 kDa, and detected with anti-chicken-HRP.

Applications for CACNA2D2 Antibody (N492/39) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CACNA2D2

The CACNA2D2 gene encodes a voltage-dependent calcium channel subunit alpha-2/delta-2 protein that exists in 5 isoforms: isoform 1: 1,150 amino acids long, 129 kDA; isoform 2: 1,143 amino acids long, 129 kDA; isoform 3: 1,145 amino acids long, 129 kDA; isoform 4: 1,076 amino acids long, 122 kDA; and isoform 5: 1,152 amino acids long, 130 kDA. These proteins are critical in calcium current density and in activation and inactivation kinetics of the calcium channel. Additionally, these proteins function as regulatory subunits for P/Q-type calcium channel, N-type, and L-type, as well as T-type. Overexpression of this gene triggers apoptosis. The CACNA2D2 gene participates in transcription of the CREB pathway, the caspase cascade, Fc-GammaR pathway, BMP and PDGF pathways, regulation of insulin secretion, and transmission across chemical synapses. It is known to interact with genes DLG4, CACNA1C, CACNA1D, CACNA1F, and YWHAB. The CACNA2D2 gene is associated with ataxia, seizures, lung cancer, and neuronitis.

Long Name

Voltage-dependent calcium channel subunit alpha-2/delta-2

Alternate Names

KIAA0558

Gene Symbol

CACNA2D2

Additional CACNA2D2 Products

Product Documents for CACNA2D2 Antibody (N492/39) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CACNA2D2 Antibody (N492/39) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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