Calcineurin A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88218

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Calcineurin A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calcineurin A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Calcineurin A Antibody [NBP1-88218]

Immunocytochemistry/ Immunofluorescence: Calcineurin A Antibody [NBP1-88218]

Immunocytochemistry/Immunofluorescence: Calcineurin A Antibody [NBP1-88218] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Calcineurin A Antibody - BSA Free Immunohistochemistry-Paraffin: Calcineurin A Antibody - BSA Free [NBP1-88218]

Immunohistochemistry-Paraffin: Calcineurin A Antibody - BSA Free [NBP1-88218]

Analysis in human cerebral cortex and pancreas tissues using HPA012778 antibody. Corresponding PPP3CA RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218] - Staining of human prostate shows moderate positivity in plasma membrane in glandular cells
Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218] - Staining of human testis shows weak membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218]

Immunohistochemistry-Paraffin: Calcineurin A Antibody [NBP1-88218] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Calcineurin A Antibody - BSA Free Western Blot: Calcineurin A Antibody - BSA Free [NBP1-88218]

Western Blot: Calcineurin A Antibody - BSA Free [NBP1-88218]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Calcineurin A Antibody - BSA Free Western Blot: Calcineurin A Antibody - BSA Free [NBP1-88218]

Western Blot: Calcineurin A Antibody - BSA Free [NBP1-88218]

Analysis in human cell line SCLC-21H.

Applications for Calcineurin A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calcineurin A

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Alternate Names

CALNA, CNA1, PP2B, PP3CA, PPP2B, PPP3CA

Gene Symbol

PPP3CA

Additional Calcineurin A Products

Product Documents for Calcineurin A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Calcineurin A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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