Calreticulin Antibody (1G6A7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80610

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1G6A7

Format

Azide and BSA Free
View all Calreticulin Primary Antibodies »

Product Specifications

Immunogen

Calreticulin Antibody (1G6A7) was developed against a synthetic peptide corresponding to the C-terminus (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH. [UniProt# P27797]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Calreticulin Antibody (1G6A7) - Azide and BSA Free

Western Blot: Calreticulin Antibody (1G6A7)Azide and BSA Free [NBP2-80610]

Western Blot: Calreticulin Antibody (1G6A7)Azide and BSA Free [NBP2-80610]

Western Blot: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - Analysis using Calreticulin mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Calreticulin cDNA (2). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Calreticulin (1G6A7) conjugated to Alexa Fluor 488 [NBP1-47518AF488] at 10 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunohistochemistry-Paraffin: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunohistochemistry-Paraffin: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - Analysis of paraffin-embedded human placenta tissues using anti-Calreticulin mAb. Image from the standard format of this antibody.
Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - An intracellular stain was performed on NIH3T3 cells with Calreticulin (1G6A7) Antibody NBP1-47518APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Allophycocyanin.
Western Blot: Calreticulin Antibody (1G6A7)Azide and BSA Free [NBP2-80610]

Western Blot: Calreticulin Antibody (1G6A7)Azide and BSA Free [NBP2-80610]

Western Blot: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - Analysis of whole cell lysates from HeLa, A549, NTERA2 and MCF-7 using Calreticulin antibody clone 1G6A7. The antibody generated a specific band of Calreticulin protein at ~48 kDa molecular weight position. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - Analysis of 3T3-L1 cells using anti-Calreticulin mAb (green). DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - Analysis of SKBR-3 (left) and A549 (right) cells using anti-Calreticulin mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunocytochemistry/ Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - Antibody was tested in HeLa cells at a 1:250 against DyLight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue). Image from the standard format of this antibody.
Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - An intracellular stain was performed on A431 cells with Calreticulin (1G6A7) Antibody NBP1-47518 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488. Image from the standard format of this antibody.
Flow Cytometry: Calreticulin Antibody (1G6A7)Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7)Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - An intracellular stain was performed on HeLa cells with Calreticulin Antibody [1G6A7] NBP1-47518AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Flow Cytometry: Calreticulin Antibody (1G6A7)Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7)Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - An intracellular stain was performed on HeLa cells with Calreticulin Antibody [1G6A7] NBP1-47518APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Allophycocyanin.
Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610]

Flow Cytometry: Calreticulin Antibody (1G6A7) - Azide and BSA Free [NBP2-80610] - An intracellular stain was performed on NIH3T3 cells with Calreticulin (1G6A7) Antibody NBP1-47518F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.

Applications for Calreticulin Antibody (1G6A7) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calreticulin

Calreticulin, also called CALR, is an endoplasmic reticulum (ER) protein that functions as a calcium (Ca2+) buffering molecular chaperone (1-3). Together with calnexin and ERp57, calreticulin has a critical role in protein folding and quality control of newly synthesized glycoproteins (1-3). Calreticulin has many diverse functions beyond Ca2+ binding, including cell adhesion, lectin binding, apoptosis, nuclear transport, antigen presentation, and immune response (1-3). Specifically, calreticulin is part of the peptide-loading complex that resides on the ER membrane and is responsible for antigen loading onto MHC class I molecules (1-3). Calreticulin protein has a theoretical molecular weight of 46 kDa and is 417 amino acids (aa) in length (1-3). The protein has three main domains: the N-terminal lectin-like domain, the proline-rich P-domain, and the acidic C-domain which is followed by an ER-retention KDEL signal sequence (1-3).

Given its role in multiple biological processes, it makes sense that calreticulin is implicated in both healthy and disease states. Studies have found that calreticulin mutations were present in patients with myeloproliferative neoplasms (MFN) and essential thrombocythaemia (ET) (4). Calreticulin expression is typically upregulated in most cancer lines, however it is downregulated in some tissues including cervical carcinomas, prostate cancer, and human colon adenocarcinoma (3). High expression of calreticulin on cancer cells is related to tumor cell phagocytosis and is often correlated with, and counteracted by, elevated CD47 expression to prevent cancer cell phagocytosis (3). Calreticulin mutations can serve as a major diagnostic biomarker for MFN and ET and additionally the calreticulin gene may be a potential target for cancer therapeutics (3,4).

References

1. Michalak, M., Groenendyk, J., Szabo, E., Gold, L. I., & Opas, M. (2009). Calreticulin, a multi-process calcium-buffering chaperone of the endoplasmic reticulum. The Biochemical Journal. https://doi.org/10.1042/BJ20081847

2. Fucikova, J., Spisek, R., Kroemer, G., & Galluzzi, L. (2021). Calreticulin and cancer. Cell Research. https://doi.org/10.1038/s41422-020-0383-9

3. Sun, J., Mu, H., Dai, K., & Yi, L. (2017). Calreticulin: a potential anti-cancer therapeutic target. Die Pharmazie. https://doi.org/10.1691/ph.2017.7031

4. Prins, D., Gonzalez Arias, C., Klampfl, T., Grinfeld, J., & Green, A. R. (2020). Mutant Calreticulin in the Myeloproliferative Neoplasms. HemaSphere. https://doi.org/10.1097/HS9.0000000000000333

Alternate Names

Autoantigen Ro, CALR, Calregulin, cC1qR, CRT, SSA, Vasostatin

Gene Symbol

CALR

Additional Calreticulin Products

Product Documents for Calreticulin Antibody (1G6A7) - Azide and BSA Free

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Product Specific Notices for Calreticulin Antibody (1G6A7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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