Canine CCL2/JE/MCP-1 Biotinylated Antibody

  
  • Species Reactivity
    Canine
  • Specificity
    Detects canine CCL2/JE/MCP‑1 in ELISAs and Western blots. In sandwich immunoassay, less than 0.05% cross‑reactivity with recombinant mouse MCP-1 and recombinant human MCP-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant canine CCL2/JE/MCP‑1
    Gln24-Pro101
    Accession # P52203
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Canine CCL2/JE/MCP‑1 (Catalog # 1774-MC)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Canine CCL2/JE/MCP-1 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Canine CCL2/JE/MCP‑1 Antibody (Catalog # MAB28171)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Canine CCL2/JE/MCP‑1 Biotinylated Antibody (Catalog # BAF1774)
  • ELISA Standard
     
    Recombinant Canine CCL2/MCP-1 Protein (Catalog # 1774-MC)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CCL2/JE/MCP‑1 in Canine PBMCs.

CCL2/JE/MCP‑1 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine CCL2/JE/MCP‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1774) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1

Canine MCP-1 (monocyte chemotactic protein-1) is an 8 kDa member of the CC chemokine family of chemotactic factors (1, 2). It is synthesized as a 101 amino acid (aa) precursor that contains a 23 aa signal sequence and a 78 aa mature segment (3). It contains no potential N-linked glycosylation sites and is not known for any posttranslational modifications. Based on human studies, MCP-1 will primarily circulate as a monomer. Noncovalent dimers are likely to be found, however. MCP‑1 activity has been localized to the N-terminus (1). Cell types known to secrete MCP-1 are considerable in number, and include keratinocytes, fibroblasts, endothelium, osteoblasts, macrophages, mast cells, smooth muscle cells and astrocytes (1, 2). In the mature MCP-1 segment, there is 82% and 83% aa identity, canine to human and porcine MCP-1, respectively. When mature canine MCP-1 is compared to (125 aa) extended rodent MCP-1, there is 55% and 56% aa identity, canine to mouse and rat MCP-1, respectively. MCP-1 has three possible receptors. The first two are CCR2 (1) and CCR11 (4). The third receptor has only been identified in mice and is called L-CCR (5). Its function is unknown. MCP-1 is best known as a chemotactic agent for mononuclear cells. It also, however, induces enzyme and cytokine release in monocytes, NK cells, and lymphocytes and histamine release by basophils (1). Additionally, it is believed to reduce IL‑12 production by dendritic cells and promote a Th2 phenotype in CD4+ T cells (6).

  • References:
    1. Coillie, E.V. et al. (1999) Cytokine Growth Factor Rev. 10:61.
    2. Yoshie, O. et al. (2001) Adv. Immunol. 78:57.
    3. Kumar, A.G. et al. (1997) Circulation 95:693.
    4. Biber, K. et al. (2003) J. Leukoc. Biol. 74:243.
    5. Luther, S.A. and J.G. Cyster  (2001) Nat. Immunol. 2:102.
  • Entrez Gene IDs:
    6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
  • Alternate Names:
    CCL2; MCAF; MCP-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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