Carbonic Anhydrase II/CA2 Antibody [HRP]

Carbonic Anhydrase II/CA2/CA2 [Bovine Erythrocytes] (Uniprot: P00921)

Human reactivity reported in scientific literature (PMID: 9336012).

Cytoplasmic

Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.

Polyclonal

Rabbit

IgG

This vial contains a relatively low volume of reagent (25 uL). To minimize loss of volume dilute 1:10 by adding 225 uL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20C or below after dilution. Avoid cycles of freezing and thawing.

Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]

Multi-step

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01% Gentamicin Sulfate

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C in the dark. Avoid freeze-thaw cycles.