Carm1 Antibody

Novus Biologicals | Catalog # NB100-56374

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Carm1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a mixture of synthetic peptides containing amino acids 45-69 and 595-608 of human PRMT4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carm1 Antibody

Knockout Validated: Carm1 Antibody [NB100-56374]

Western Blot: Carm1 Antibody [NB100-56374]

Western Blot: Carm1 Antibody [NB100-56374] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB100-56374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: Carm1 Antibody [NB100-56374]

Western Blot: Carm1 Antibody [NB100-56374]

Western Blot: Carm1 Antibody [NB100-56374] - Analysis of PRMT4 in cell lysates from HeLa cells using this antibody. 5 ug/ml. Twenty ug of HeLa cell lysate was loaded per well of mini gel.
Immunocytochemistry/ Immunofluorescence: Carm1 Antibody [NB100-56374]

Immunocytochemistry/ Immunofluorescence: Carm1 Antibody [NB100-56374]

Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NB100-56374] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Carm1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Carm1 Antibody [NB100-56374]

Immunohistochemistry-Paraffin: Carm1 Antibody [NB100-56374]

Immunohistochemistry-Paraffin: Carm1 Antibody [NB100-56374] - Analysis of a FFPE tissue section of the human breast carcinoma using 1:200 dilution of Carm1 antibody (NB100-56374). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated nuclear and cytoplasmic staining.

Applications for Carm1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carm1

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. CARM1 (coactivator-associated arginine methyltransferase-1) also known as PRMT4 (protein-arginine N-methyltransferase-4) belongs to a family of proteins that catalyzes the methylation of arginine residues. The PRMT proteins are recruited by C-terminal activation domain-2 of the SRC (steroid receptor coactivator) GRIP-1, whereas p300 interacts with activation domain-1 in the C terminus. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduces both methyltransferase and coactivator activities suggesting that coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.

Long Name

Coactivator-associated Arginine Methyltransferase 1

Alternate Names

PRMT4

Entrez Gene IDs

10498 (Human)

Gene Symbol

CARM1

UniProt

Q86X55 (Human)

Additional Carm1 Products

Product Documents for Carm1 Antibody

Certificate of Analysis

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Product Specific Notices for Carm1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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