Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71948

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D9

Format

Azide and BSA Free
View all Casein Kinase 1 gamma Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 163-422 of human CSNK1G1(NP_071331) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free

Western Blot: Casein Kinase 1 gamma Antibody (OTI4D9)Azide and BSA Free [NBP2-71948]

Western Blot: Casein Kinase 1 gamma Antibody (OTI4D9)Azide and BSA Free [NBP2-71948]

Western Blot: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Casein Kinase 1 gamma.
Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948]

Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948]

Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948]

Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948]

Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948]

Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948]

Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948]

Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948]

Immunohistochemistry: Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free [NBP2-71948] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Casein Kinase 1 gamma

Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/ threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIalpha, gamma, episilon and delta, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an alpha2beta2 or an alphaalpha'beta2 structure. The alpha catalytic subunit is stimulated by the beta regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Alternate Names

CSNK1G1

Gene Symbol

CSNK1G1

Additional Casein Kinase 1 gamma Products

Product Documents for Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Casein Kinase 1 gamma Antibody (OTI4D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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Protocols

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FAQs

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