Caspase-2 Antibody [CoraFluor™ 1]

Novus Biologicals | Catalog # NBP2-26612CL1

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG
View all Caspase-2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-2. The immunogen is located within the first 50 amino acids of Caspase-2.

Specificity

Depending on cell lines or tissues used, other cleavage products may be observed

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for Caspase-2 Antibody [CoraFluor™ 1]

Caspase-2 Antibody [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for Caspase-2 Antibody [CoraFluor™ 1]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-26612. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Caspase-2

Caspase-2, also known as NEDD2 and ICH-1, is a 30‑32 kDa member of the peptidase C14A/IL‑1 beta‑converting family of enzymes. It is widely expressed and is an integral component of the apoptotic cascade. Caspase-2 is a disulfide-linked homodimer, with activation occurring following oligomerization within the context of activating platforms such as DISC (death-inducing signaling complex) or the PIDDosome. Initially, Pro-Caspase-2 is cleaved into a p34 subunit and a p14 subunit which are further processed to generate the prodomain, plus the mature p18 and p12 subunits. Notably, each p18-p12 heterodimer demonstrates proteolytic activity and localizes to either the nucleus or cytoplasm.

 

Alternate Names

CASP2, Caspase2, ICH-1, NEDD2

Gene Symbol

CASP2

Additional Caspase-2 Products

Product Documents for Caspase-2 Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Caspase-2 Antibody [CoraFluor™ 1]

Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail