Caspase-8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76610

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caspase-8 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A. The immunogen is located within the last 50 amino acids of Caspase-8. Amino Acid Squence: NYEVSNKDDKKNMGKQ

Specificity

Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-8 Antibody - BSA Free

Western Blot: Caspase-8 AntibodyBSA Free [NBP1-76610]

Western Blot: Caspase-8 AntibodyBSA Free [NBP1-76610]

Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 ug/ml.
Immunohistochemistry: Caspase-8 Antibody - BSA Free [NBP1-76610]

Immunohistochemistry: Caspase-8 Antibody - BSA Free [NBP1-76610]

Immunohistochemistry: Caspase-8 Antibody [NBP1-76610] - Staining of human spleen tissue with antibody at 5 ug/mL.
Western Blot: Caspase-8 AntibodyBSA Free [NBP1-76610]

Western Blot: Caspase-8 AntibodyBSA Free [NBP1-76610]

Western Blot: Caspase-8 Antibody [NBP1-76610] - Analysis in Jurkat cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610]

Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610]

Immunocytochemistry/Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - Jurkat cells with caspase-8 antibody at 2 ug/ml.
Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610]

Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610]

Immunofluorescence: Caspase-8 Antibody [NBP1-76610] - IF of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 ug/ml. Red: Caspase-8 Antibody (NBP1-76610). Blue: DAPI staining
Caspase-8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610] -

Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody - BSA Free [NBP1-76610] - Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 ug/mL.

Applications for Caspase-8 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Caspase-8

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-8 is an initiator caspase that was identified as a member of the Fas/APO-1 death-inducing signaling complex. The adaptor molecule FADD couples procaspase-8 to the Fas receptor death domain; subsequent oligomerization promotes procaspase-8 autoactivation. FLIP, a catalytically inactive caspase-8-like molecule inhibits these interactions and thus can inhibit apoptosis.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

UniProt

NP_001219 (Human)

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caspase-8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Caspase-8 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-8 Antibody - BSA Free

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  • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

    A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail