Caspase-8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15722

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caspase-8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase-8. The exact sequence is proprietary.

Localization

Cytoplasm;

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-8 Antibody - BSA Free

Western Blot: Caspase-8 Antibody [NBP2-15722]

Western Blot: Caspase-8 Antibody [NBP2-15722]

Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Caspase-8 Antibody [NBP2-15722]

Western Blot: Caspase-8 Antibody [NBP2-15722]

Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Caspase-8 Antibody

Western Blot: Caspase-8 Antibody [NBP2-15722] -

Western Blot: Caspase-8 Antibody [NBP2-15722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase-8 antibody (NBP2-15722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Caspase-8 Antibody

Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] -

Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Caspase-8 Antibody

Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] -

Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: Caspase-8 protein stained by Caspase-8 antibody (NBP2-15722) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Caspase-8 Antibody

Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] -

Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:100.
Blue: Hoechst 33342 staining.
Caspase-8 Antibody

Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] -

Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Caspase-8 Antibody

Western Blot: Caspase-8 Antibody [NBP2-15722] -

Western Blot: Caspase-8 Antibody [NBP2-15722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase-8 antibody (NBP2-15722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Caspase-8 Antibody - BSA Free

Western Blot: Caspase-8 Antibody [NBP2-15722] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (NBP2-15722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Caspase-8 Antibody - BSA Free

Western Blot: Caspase-8 Antibody [NBP2-15722] -

Wild-type (WT) and CASP8 (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody (NBP2-15722) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Caspase-8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Caspase-8

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq]

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

UniProt

Q14790

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Caspase-8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Caspase-8 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-8 Antibody - BSA Free

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  • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

    A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail