Caspase-9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76961

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Caspase-9 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 20 amino acids near the center of human Caspase 9. The immunogen is located within amino acids 290-340 of Caspase 9. Amino Acid Sequence: ASTSPEDESPGSNPEPDATP

Specificity

Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family. Human CASPASE 9 has 4 isoforms, including isoform 1 (416aa, 46kD), isoform 2 (266aa, 30kD), isoform 3 (154aa, 17kD), and isoform 4 (333aa, 37kD). Mouse CASPASE 9 has one isoform (454aa, 50kD) and Rat CASPASE 9 also has one isoform (454aa, 50kD). NBP1-76961 can detect human and mouse isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Caspase-9 Antibody - BSA Free

Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Mouse Cell Line. Loading: 15 ug of 3T3/NIH cell lysate.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 Antibody [NBP1-76961] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Caspase 9, NBP1-76470 (1 ug/mL), Caspase 9, NBP1-76961 (1 ug/mL) and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 AntibodyBSA Free [NBP1-76961]

Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Caspase-9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] -

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] - Validation of Caspase 9 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Caspase-9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] -

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] - Immunofluorescence Validation of Caspase 9 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
Caspase-9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] -

Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] - Validation of Caspase 9 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-Caspase 9 antibody at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Applications for Caspase-9 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry

2- 5 ug/mL

Immunocytochemistry/ Immunofluorescence

2-20 ug/mL

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-9

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFalpha, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Alternate Names

CASP9, Caspase9

Gene Symbol

CASP9

UniProt

P55211

Additional Caspase-9 Products

Product Documents for Caspase-9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Caspase-9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Citations for Caspase-9 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-9 Antibody - BSA Free

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  • Q: Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?

    A: After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

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