Catalase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38772

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Catalase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNFTEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Catalase Antibody - BSA Free

Western Blot: Catalase Antibody [NBP2-38772]

Western Blot: Catalase Antibody [NBP2-38772]

Western Blot: Catalase Antibody [NBP2-38772] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: Catalase Antibody [NBP2-38772]

Immunocytochemistry/ Immunofluorescence: Catalase Antibody [NBP2-38772]

Immunocytochemistry/Immunofluorescence: Catalase Antibody [NBP2-38772] - Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772] - Staining of human pancreas using Anti-CAT antibody NBP2-38772.
Immunohistochemistry: Catalase Antibody [NBP2-38772]

Immunohistochemistry: Catalase Antibody [NBP2-38772]

Immunohistochemistry: Catalase Antibody [NBP2-38772] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772] - Staining in human liver and pancreas tissues using anti-CAT antibody. Corresponding CAT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772] - Staining of human bone marrow, liver, lymph node and pancreas using Anti-CAT antibody NBP2-38772 (A) shows similar protein distribution across tissues to independent antibody NBP2-38646 (B).
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772] - Staining of human bone marrow.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772] - Staining of human lymph node.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772]

Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-38772] - Staining of human liver using Anti-CAT antibody NBP2-38772.

Applications for Catalase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Catalase

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Alternate Names

Cas1, CAT, Cs-1

Gene Symbol

CAT

UniProt

P04040

Additional Catalase Products

Product Documents for Catalase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Catalase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Oxidative Stress

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Protocols

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