CBP/KAT3A Antibody (2H5) - Azide and BSA Free

Novus Biologicals | Catalog # H00001387-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2H5

Format

Azide and BSA Free
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Product Specifications

Immunogen

CREBBP (NP_004371, 951 a.a. ~ 1050 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PVHAQPPGTPLSQAAASIDNRVPTPSSVASAETNSQQPGPDVPVLEMKTETQAEDTEPDPGESKGEPRSEMMEEDLQGASQVKEETDIAEQKSEPMEVDE

Specificity

CREBBP - CREB binding protein (Rubinstein-Taybi syndrome)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CBP/KAT3A Antibody (2H5) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CBP/KAT3A Antibody (2H5) [H00001387-M01]

Immunocytochemistry/ Immunofluorescence: CBP/KAT3A Antibody (2H5) [H00001387-M01]

Immunocytochemistry/Immunofluorescence: CBP/KAT3A Antibody (2H5) [H00001387-M01] - Analysis of monoclonal antibody to CREBBP on HeLa cell. Antibody concentration 10 ug/ml.
Proximity Ligation Assay: CBP/KAT3A Antibody (2H5) [H00001387-M01]

Proximity Ligation Assay: CBP/KAT3A Antibody (2H5) [H00001387-M01]

Proximity Ligation Assay: CBP/KAT3A Antibody (2H5) [H00001387-M01] - Protein-protein interactions between FGFR1 and CREBBP HeLa cells were stained with anti-FGFR1 (H00002260-D01P) rabbit purified polyclonal 1:1200 and anti-CREBBP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: CBP/KAT3A Antibody (2H5) [H00001387-M01] - Detection limit for recombinant GST tagged CREBBP is approximately 0.03ng/ml as a capture antibody.

Applications for CBP/KAT3A Antibody (2H5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CBP

The CBP (CREB-binding Protein) is a histone acetyltransferase and transcriptional coactivator that regulates RNA polymerase II transcription. In addition to histone acetylation, CBP also acetylates various transcription factors, including the tumor suppressor protein p53, to enhance sequence-specific DNA binding.

Long Name

CREB-binding Protein

Alternate Names

CREBBP, RTS

Entrez Gene IDs

1387 (Human)

Gene Symbol

CREBBP

UniProt

NP_004371 (Human)

Additional CBP Products

Product Documents for CBP/KAT3A Antibody (2H5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CBP/KAT3A Antibody (2H5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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