CCDC90B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-93529

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MNSRQAWRLLLSQGRGDRWVSRPRGHFSPALRREFFTTTTKEGYDRRPVDITPLEQRKLTFDTHALVQDLETHGFDKTQAETIVSALTALSNVSLDTIYKEMVTQAQQEITVQQL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%). Reactivity reported in scientific literature (PMID: 23435261)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCDC90B Antibody - BSA Free

Western Blot: CCDC90B Antibody [NBP1-93529]

Western Blot: CCDC90B Antibody [NBP1-93529]

Western Blot: CCDC90B Antibody [NBP1-93529] - Analysis using Anti-CCDC90B antibody NBP1-93529 (A) shows similar pattern to independent antibody NBP1-93528 (B).
Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529]

Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529]

Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529] - Staining in human testis and skin tissues using anti-CCDC90B antibody. Corresponding CCDC90B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529]

Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529]

Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529]

Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529]

Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529]

Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529]

Immunohistochemistry-Paraffin: CCDC90B Antibody [NBP1-93529] - Staining of human testis shows high expression.
CCDC90B Antibody - BSA Free Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.
CCDC90B Antibody - BSA Free Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Staining of human skin shows moderate granular cytoplasmic positivity in squamous epithelial cells.
CCDC90B Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: CCDC90B Antibody - BSA Free [NBP1-93529]

Immunocytochemistry/ Immunofluorescence: CCDC90B Antibody - BSA Free [NBP1-93529]

Staining of human cell line U-2 OS shows localization to mitochondria.
CCDC90B Antibody - BSA Free Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Staining of human pancreas shows strong granular cytoplasmic positivity in exocrine glandular cells.
CCDC90B Antibody - BSA Free Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.
CCDC90B Antibody - BSA Free Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Immunohistochemistry: CCDC90B Antibody - BSA Free [NBP1-93529]

Analysis in human testis and skin tissues using NBP1-93529 antibody. Corresponding CCDC90B RNA-seq data are presented for the same tissues.

Applications for CCDC90B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCDC90B

Alternate Names

coiled-coil domain containing 90B, mitochondrial

Gene Symbol

Ccdc90b

Additional CCDC90B Products

Product Documents for CCDC90B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CCDC90B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCDC90B Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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