CCM2 Antibody

Novus Biologicals | Catalog # NB100-53812

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCM2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KGEKSRDKKAHEK, from the internal region of the protein sequence according to NP_001025006.1; NP_113631.1; NP_001161407.1; NP_001350387.1.

Specificity

This antibody is expected to recognize isoform 1 (NP_001025006.1), isoform 2 (NP_113631.1) and isoform 4 (NP_001161407.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CCM2 Antibody

CCM2 Antibody

Flow Cytometry: CCM2 Antibody [NB100-53812] -

Flow Cytometry: CCM2 Antibody [NB100-53812] - Flow cytometric analysis of paraformaldehyde fixed KNRK cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
CCM2 Antibody

Western Blot: CCM2 Antibody [NB100-53812] -

Western Blot: CCM2 Antibody [NB100-53812] - (1ug/ml) staining of Rat KNRK cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
CCM2 Antibody

Immunocytochemistry/Immunofluorescence: CCM2 Antibody [NB100-53812] -

Immunocytochemistry/Immunofluorescence: CCM2 Antibody [NB100-53812] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
CCM2 Antibody

Western Blot: CCM2 Antibody [NB100-53812] -

Western Blot: CCM2 Antibody [NB100-53812] - (0.5ug/ml) staining of Human Heart (A) and (2ug/ml) Cerebral Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for CCM2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3-2 ug/ml
Application Notes
WB: Approx 45-48kDa band observed in Human Heart lysates and approx.48kDa in Human Cerebral Cortex lysates and in lysates of cell line KNRK (calculated MW of 48.8kDa according to Human NP_113631.1 and 49.8kDa according to Rat NP_001119747.1). Primary incubation 1 hour at room temperature.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CCM2

p16 INK4a is a tumor suppressor protein. It is a specific inhibitor of Cdk 4 / Cdk 6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Recent analyses of the p16 INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. Although the frequency of p16 INK4a abnormalities is higher in tumor derived cell lines than in unselected primary tumors, significant subsets of clinical cases with aberrant p16 INK4a gene have been reported among melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladder carcinomas, and some types of leukemia.

Alternate Names

C7orf22OSM, cerebral cavernous malformation 2, Cerebral cavernous malformations 2 protein, chromosome 7 open reading frame 22, malcavernin, MGC4067, MGC4607, MGC74868

Entrez Gene IDs

83605 (Human); 216527 (Mouse)

Gene Symbol

CCM2

UniProt

NP_001025006.1 (Human)

Additional CCM2 Products

Product Documents for CCM2 Antibody

Certificate of Analysis

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Product Specific Notices for CCM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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