CCR4 Antibody (HL2481) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25402
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2481 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Synthetic peptide of human CCR4.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CCR4 Antibody (HL2481) - Azide and BSA Free
Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] -
Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] -
Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat thymus gland. CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] -
Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] -
Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Non-transfected (-) and transfected (+) boiled and unboiled SW480 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] -
Western Blot: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - Unboiled SW480 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)
Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] -
Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) - Azide and BSA Free [NBP3-25402] - CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: CCR4 Antibody (HL2481) [NBP3-25402] -
CCR4 antibody [HL2481] detects CCR4 protein by immunofluorescent analysis.Sample: SW480 cells were fixed in ice-cold MeOH for 5 min.
Green: CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:200.
Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) [NBP3-25402] -
CCR4 antibody [HL2481] detects CCR4 protein by immunohistochemical analysis.Sample: Paraffin-embedded human tonsil.
CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: CCR4 Antibody (HL2481) [NBP3-25402] -
Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: CCR4 Antibody (HL2481) [NBP3-25402] -
CCR4 antibody [HL2481] detects CCR4 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.
CCR4 stained by CCR4 antibody [HL2481] (NBP3-25402) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for CCR4 Antibody (HL2481) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCR4
Alternate Names
CD194
Gene Symbol
CCR4
Additional CCR4 Products
Product Documents for CCR4 Antibody (HL2481) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CCR4 Antibody (HL2481) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
