CCRL2/CRAM-A/B Antibody (BZ2E3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-11983

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional Assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2a Kappa Clone # BZ2E3

Format

Azide and BSA Free
View all CCRL2/CRAM-A/B Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising Wistar Furth rats with the mCCRL2/CRAM-A/B peptide/KLH conjugate. The immunizing amino-terminal mCCRL2/CRAM-A/B peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2/CRAM-A/B, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).

Specificity

This antibody is specific for murine CCRL2/CRAM-A/B (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2/CRAM-A/B presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2/CRAM-A/B antibody binding.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Applications for CCRL2/CRAM-A/B Antibody (BZ2E3) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional Assay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CCRL2/CRAM-A/B

CCRL2 encodes a chemokine receptor like protein, which is predicted to be a seven transmembrane protein and most closely related to CCR1. Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. This gene is expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. The function of this gene is unknown. This gene is mapped to the region where the chemokine receptor gene cluster is located. [provided by RefSeq]

Long Name

CC Chemokine Receptor-like 2

Alternate Names

ACKR5, CCRL2, CKRX, CRAM-A, CRAM-B, CRAMA, CRAMB, HCR

Gene Symbol

CCRL2

Additional CCRL2/CRAM-A/B Products

Product Documents for CCRL2/CRAM-A/B Antibody (BZ2E3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CCRL2/CRAM-A/B Antibody (BZ2E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CCRL2/CRAM-A/B Antibody (BZ2E3) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review CCRL2/CRAM-A/B Antibody (BZ2E3) - Azide and BSA Free and earn rewards!

Have you used CCRL2/CRAM-A/B Antibody (BZ2E3) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies