CCT5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83042

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CCT5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CCT5 Antibody - BSA Free

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining in human testis and liver tissues using anti-CCT5 antibody. Corresponding CCT5 RNA-seq data are presented for the same tissues.
Western Blot: CCT5 Antibody [NBP1-83042]

Western Blot: CCT5 Antibody [NBP1-83042]

Western Blot: CCT5 Antibody [NBP1-83042] - Analysis using Anti-CCT5 antibody NBP1-83042 (A) shows similar pattern to independent antibody NBP1-83043 (B).
Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human cerebral cortex, colon, liver and testis using Anti-CCT5 antibody NBP1-83042 (A) shows similar protein distribution across tissues to independent antibody NBP1-83043 (B).
Immunocytochemistry/ Immunofluorescence: CCT5 Antibody [NBP1-83042]

Immunocytochemistry/ Immunofluorescence: CCT5 Antibody [NBP1-83042]

Immunocytochemistry/Immunofluorescence: CCT5 Antibody [NBP1-83042] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human cerebral cortex.
Western Blot: CCT5 Antibody [NBP1-83042]

Western Blot: CCT5 Antibody [NBP1-83042]

Western Blot: CCT5 Antibody [NBP1-83042] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042]

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-83042] - Staining of human colon.

Applications for CCT5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCT5

CCT5 encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Alternate Names

chaperonin containing TCP1, subunit 5 (epsilon), epsilon subunit

Gene Symbol

CCT5

Additional CCT5 Products

Product Documents for CCT5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CCT5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCT5 Antibody - BSA Free

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Protocols

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