CD117/c-kit Antibody (KIT/982)

Name: CD117/c-kit Antibody (KIT/982)
Brand: Novus Biologicals
SKU: NBP2-44761
Price: 209 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-44761

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # KIT/982

View all CD117/c-kit Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa100-300) of human CD117/c-kit protein (exact sequence is proprietary) (Uniprot: P10721)

Localization

Cell surface and cytoplasmic

Marker

Marker for Gastrointestinal Stromal Tumors

Specificity

This monoclonal antibody recognizes a protein of 145kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposis sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Theoretical MW

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11446)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD117/c-kit Antibody (KIT/982)

Western Blot: CD117/c-kit Antibody (KIT/982) [NBP2-44761]

Western Blot: CD117/c-kit Antibody (KIT/982) [NBP2-44761] - Recombinant fragment of CD117 with CD117 Monoclonal Antibody (KIT/982).

Protein Array: CD117/c-kit Antibody (KIT/982) [NBP2-44761]

Protein Array: CD117/c-kit Antibody (KIT/982) [NBP2-44761] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD117/c-kit Antibody (KIT/982). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.

Applications for CD117/c-kit Antibody (KIT/982)

Application

Recommended Usage

Western Blot

1-2 ug/ml

Application Notes

Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 2 hours at RT.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD117/c-kit

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Long Name

Stem Cell Factor Receptor

Alternate Names

c-kit, CD117, ckit, KIT, PBT, SCF R, SCFR

Gene Symbol

KIT

UniProt

P10721

Additional CD117/c-kit Products

Product Documents for CD117/c-kit Antibody (KIT/982)

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Certificate of Analysis

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Product Specific Notices for CD117/c-kit Antibody (KIT/982)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.