CD11c Antibody (BLR138H)

Name: CD11c Antibody (BLR138H)
Brand: Novus Biologicals
SKU: NBP3-14718
Price: 539 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-14718

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, COMET

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR138H

View all CD11c Primary Antibodies »

Product Specifications

Immunogen

residues 112-355 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for CD11c Antibody (BLR138H)

Detection of CD11c in Human Tonsil via seqIF™ Staining on COMET™

CD11c was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human CD11c Monoclonal Antibody (Catalog # NBP3-14718) at 1:80 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.

Western Blot: CD11c Antibody (BLR138H) [NBP3-14718]

Western Blot: CD11c Antibody (BLR138H) [NBP3-14718] - Whole cell lysate (50 ug) from THP-1, HeLa, HEK293T, KG-1, Jurkat, Hep-G2, MCF-7, and NK-92 cells prepared using NETN lysis buffer. Rabbit anti-CD11c recombinant monoclonal antibody used at 1:1000. HRP-conjugated goat anti-rabbit IgG Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718]

Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718] - Rabbit anti-CD11c recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718]

Immunohistochemistry: CD11c Antibody (BLR138H) [NBP3-14718] - Whole cell lysate (50 ug) from THP-1, HeLa, HEK293T, KG-1, Jurkat, Hep-G2, MCF-7, and NK-92 cells prepared using NETN lysis buffer. Rabbit anti-CD11c recombinant monoclonal antibody used at 1:1000.

ICC/IF: CD11c Antibody (BLR138H) [NBP3-14718] -

ICC/IF: CD11c Antibody (BLR138H) [NBP3-14718] - Rabbit antiCD11c recombinant monoclonal antibody [BLR138H]. Secondary: HRP-conjugated goat antirabbit IgG. Substrate: DAB.

View 2 more images

Applications for CD11c Antibody (BLR138H)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Multiplex Immunofluorescence

1:80

Western Blot

1:1,000

Application Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD11c

CD11c encodes the integrin alpha X chain protein. CD11c is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Alternate Names

CD11c, Integrin alpha X, ITGAX, p150,95 alpha

Gene Symbol

ITGAX

Additional CD11c Products

Product Documents for CD11c Antibody (BLR138H)

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD11c Antibody (BLR138H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.