CD133 Antibody (3F10)

Novus Biologicals | Catalog # NBP2-37741

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3F10
View all CD133 Primary Antibodies »

Product Specifications

Immunogen

Synthesized peptide of human CD133.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33814238).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD133 Antibody (3F10)

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human esophageal cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.
Immunohistochemistry-Paraffin: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human breast cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated secondary antibody and DAB method which followed counterstaining of nuclei with hematoxylin.
Flow Cytometry: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Flow Cytometry: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Flow Cytometry: CD133 Antibody (3F10) [NBP2-37741] - Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).
ELISA: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

ELISA: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

ELISA: CD133 Antibody (3F10) [NBP2-37741] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Immunocytochemistry/ Immunofluorescence: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

Immunocytochemistry/ Immunofluorescence: CD133 Antibody (3F10) - BSA Free [NBP2-37741]

CD133-Antibody-3F10-Immunocytochemistry-Immunofluorescence-NBP2-37741-img0007.jpg
CD133 Antibody (3F10) - BSA Free

Immunocytochemistry/ Immunofluorescence: CD133 Antibody (3F10) - BSA Free [NBP2-37741] -

Immunocytochemistry/ Immunofluorescence: CD133 Antibody (3F10) - BSA Free [NBP2-37741] - Significant correlations between levels of TIPRL, LC3 & CD133 in liver cancers. (a–c) The statistically significant associations between levels of TIPRL, LC3 & CD133 in each grade of liver cancers were determined by the Spearman correlation. Each dot indicates a single patient. (d) The representative images were selected from each grade of human liver cancer tissues that were stained with indicated antibodies. DAPI was used for nucleus staining, & scar bar, 100 μm. (e) Quantitative analyses of TIPRL, LC3 & CD133 were conducted according to each grade of liver cancers. (n), the number of samples is indicated. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31727942), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CD133 Antibody (3F10)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200-1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30739275).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (3F10)

Certificate of Analysis

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Download SDS

Product Specific Notices for CD133 Antibody (3F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD133 Antibody (3F10)

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Protocols

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