CD133 Antibody (CL7971) - BSA Free

Novus Biologicals | Catalog # NBP3-21163

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL7971

Format

BSA Free
View all CD133 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from O43490, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD133 Antibody (CL7971) - BSA Free

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Staining of human endometrium shows moderate positivity in apical membrane in glandular cells.
Western Blot: CD133 Antibody [NBP3-21163] -

Western Blot: CD133 Antibody [NBP3-21163] -

Analysis in human cell line NTERA-2.
Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Staining of human colorectal cancer shows strong membranous positivity.
Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Staining of human pancreas shows strong membranous positivity in intercalated ducts.
Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.
Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-21163] -

Staining of human kidney shows moderate positivity in Bowman's capsule.

Applications for CD133 Antibody (CL7971) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (CL7971) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD133 Antibody (CL7971) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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