CD133 Antibody (CL7971) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44257

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL7971

Format

Azide and BSA Free
View all CD133 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of O43490, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse CD133 Antibody (CL7971) - Azide and BSA Free (NBP3-21163) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD133 Antibody (CL7971) - Azide and BSA Free

CD133 Antibody (CL7971) - Azide and BSA Free Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Staining of human Fallopian tube shows strong positivity in cilia in glandular cells.
CD133 Antibody (CL7971) - Azide and BSA Free Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Staining of human kidney shows moderate positivity in Bowman's capsule.
CD133 Antibody (CL7971) - Azide and BSA Free Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Staining of human colorectal cancer shows strong membranous positivity.
CD133 Antibody (CL7971) - Azide and BSA Free Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Staining of human endometrium shows moderate positivity in apical membrane in glandular cells.
CD133 Antibody (CL7971) - Azide and BSA Free Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Staining of human prostate shows no positivity in glandular cells as expected.
CD133 Antibody (CL7971) - Azide and BSA Free Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Immunohistochemistry-Paraffin: CD133 Antibody [NBP3-44257]

Staining of human pancreas shows strong membranous positivity in intercalated ducts.
CD133 Antibody (CL7971) - Azide and BSA Free Western Blot: CD133 Antibody [NBP3-44257]

Western Blot: CD133 Antibody [NBP3-44257]

Analysis in human cell line NTERA-2.

Applications for CD133 Antibody (CL7971) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD133

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Alternate Names

AC133, CD133, PROM1, Prominin 1, PROML1

Gene Symbol

PROM1

Additional CD133 Products

Product Documents for CD133 Antibody (CL7971) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD133 Antibody (CL7971) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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