CD163 Antibody (K20-T)

Novus Biologicals | Catalog # NBP1-30148

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # K20-T
View all CD163 Primary Antibodies »

Product Specifications

Immunogen

Peptide derived from N-terminal sequence of human CD163. Antibody recognizes the epitope between Gly134 - Gly148.

Epitope

Gly134 - Gly148

Marker

Histiocytic Marker

Specificity

This antibody is specific for the N terminus of CD163.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for CD163 Antibody (K20-T)

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - CD163 immunolabelling on a FFPE tissue section of human HCV liver biopsy. Image provided by Victoria Gadd.
Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - CD163 expression in the macrophages of the bone marrow. FFPE human tissues (4 um sections) stained with anti-CD163.
Immunocytochemistry/ Immunofluorescence: CD163 Antibody (K20-T) [NBP1-30148]

Immunocytochemistry/ Immunofluorescence: CD163 Antibody (K20-T) [NBP1-30148]

Immunocytochemistry/Immunofluorescence: CD163 Antibody (K20-T) [NBP1-30148] - Human primary macrophages in 3D cell culture. Primary antibodies to CD163 (NBP1-30148; RED) and CD206 (clone 15-2; GREEN) were both diluted at 1:100 in blocking buffer and incubated overnight at 4C. This image represents a 100-micron Z-Stack displayed as maximum intensity projection. Image from verified customer review.
Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - Formalin fixed, paraffin embedded human tissues (4 um sections) stained with CD163 Antibody (K20-T).
Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - Human bone marrow.
Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148]

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - CD163-positive macrophages in the abscess of the liver. FFPE human tissues (4 um sections) stained with anti-CD163

Applications for CD163 Antibody (K20-T)

Application
Recommended Usage

Immunohistochemistry

1:100 -1:200

Immunohistochemistry-Paraffin

1:100 - 1:200
Application Notes
Use in Immunohistochemistry-Frozen was reported in scientific literature (PMID: 24798518).

Reviewed Applications

Read 2 reviews rated 5 using NBP1-30148 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris-HCl (pH 8.0) and 20mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD163

CD163 (Cluster of Differentiation 163), also known by several other names including M130, p155, RM3/1, Ki-M8, Ber-MAC3, SM4, and GHI/61, is a type I transmembrane glycoprotein that is a member of the scavenger receptor cysteine-rich (SRCR) super family class B (1-3). CD163 is expressed specifically on the monocyte/macrophage lineage and has a theoretical molecular weight of 130-160 kDa in reducing conditions and 110 kDa in non-reducing conditions (2 - 6). CD163 is synthesized as 1076 amino acid (aa) protein consisting of a large extracellular domain (ECD) with nine SRCR domains, proline-serine-threonine rich (PST) linker domain, a transmembrane segment, and a cytoplasmic tail which has varying lengths depending on the isoform due to alternative splicing, with the short 49 aa form being the most common (1-3, 6).

One of the primary functions of CD163 is uptake of haptoglobin-hemoglobin (Hp-Hb) complexes from the liver, spleen, and bone marrow, ultimately triggering an anti-inflammatory response (3, 5, 7). CD163 also functions as an erythroblast adhesion receptor and promotes cell maturation and survival (3, 5, 7). Furthermore, CD163 functions in immune sensing of bacteria and as a receptor for tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) (3, 5, 7). As mentioned above, CD163 is expressed on cells in the monocyte/macrophage lineage and, in general, anti-inflammatory signals including glucocorticoids, interleukin (IL)-6, and IL-10 stimulate CD163 synthesis and expression while, conversely, pro-inflammatory signals such as interferon-gamma (INF-gamma), TNF-alpha, and lipopolysaccharide (LPS) downregulate CD163 (3, 5). In addition to membrane-bound form of CD163, the protein can be cleaved by metalloproteinases (MMP) and induced by LPS or phorbol myristate acetate (PMA) to release a soluble form (sCD163) into the plasma (7). Increased levels of sCD163 in the plasma and an increased number of CD163-expressing macrophages at the site of inflammation are associated with a variety of pathologies (3, 5-7). CD163/sCD163 is often increased and a suitable clinical marker for inflammatory diseases including rheumatoid arthritis (RA), Gaucher disease, chronic kidney disease, diabetes, and Crohn's disease (3, 5-7).

Alternative names for CD163 includes GHI/61, HbSR, Hemoglobin scavenger receptor, M130, macrophage-associated antigen, MM130, RM3/1, SCARI1, scavenger receptor cysteine-rich type 1 protein M130, sCD163, and soluble CD163.

References

1. Law, S. K., Micklem, K. J., Shaw, J. M., Zhang, X. P., Dong, Y., Willis, A. C., & Mason, D. Y. (1993). A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. European journal of immunology. https://doi.org/10.1002/eji.1830230940

2. Onofre, G., Kolackova, M., Jankovicova, K., & Krejsek, J. (2009). Scavenger receptor CD163 and its biological functions. Acta medica (Hradec Kralove).

3. Van Gorp, H., Delputte, P. L., & Nauwynck, H. J. (2010). Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy. Molecular immunology. https://doi.org/10.1016/j.molimm.2010.02.008

4. Sulahian, T. H., Hogger, P., Wahner, A. E., Wardwell, K., Goulding, N. J., Sorg, C., Droste, A., Stehling, M., Wallace, P. K., Morganelli, P. M., & Guyre, P. M. (2000). Human monocytes express CD163, which is upregulated by IL-10 and identical to p155. Cytokine. https://doi.org/10.1006/cyto.2000.0720

5. Etzerodt, A., & Moestrup, S. K. (2013). CD163 and inflammation: biological, diagnostic, and therapeutic aspects. Antioxidants & redox signaling. https://doi.org/10.1089/ars.2012.4834

6. Skytthe, M. K., Graversen, J. H., & Moestrup, S. K. (2020). Targeting of CD163+ Macrophages in Inflammatory and Malignant Diseases. International journal of molecular sciences, 21(15), 5497. https://doi.org/10.3390/ijms21155497

7. Moller H. J. (2012). Soluble CD163. Scandinavian journal of clinical and laboratory investigation. https://doi.org/10.3109/00365513.2011.626868

Alternate Names

CD163, GHI/61, HbSR, M130, RM3/1

Entrez Gene IDs

9332 (Human)

Gene Symbol

CD163

UniProt

Q86VB7

Additional CD163 Products

Product Documents for CD163 Antibody (K20-T)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD163 Antibody (K20-T)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD163 Antibody (K20-T)

Customer Reviews for CD163 Antibody (K20-T) (2)

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Customer Images

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  • CD163 Antibody (K20-T)
    Name: Fabio Henrique Brasil da Costa
    Application: Immunocytochemistry
    Sample Tested: Human primary macrophages and 3D Cell culture
    Species: Human
    Verified Customer | Posted 11/26/2018
    Primary antibodies to CD163 (NBP1-30148; RED) and CD206 (clone 15-2; GREEN) were both diluted at 1:100 in blocking buffer and incubated overnight at 4C. This image represents a 100-micron Z-Stack displayed as maximum intensity projection.
    IF of human primary macrophages, polarized on dishes to M2 phenotype for 7 days with M-CSF and IL-4, 10 and 13. Then, cells were harvested and encapsulated into hyaluronic acid and collagen type I hydrogels and cultured for an additional 5 days, on which they were fixed with 4% PFA. Blocked in 10% goat serum, 1% BSA.This data is supported by qRT-PCR analysis of CD163 expression in another group of cells that received the same treatment. All compared to unpolarized control cells.
    CD163 Antibody (K20-T) NBP1-30148
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested:
    Species: Human
    Verified Customer | Posted 03/04/2013
    CD163 immunolabelling on a representative HCV liver biopsy section
    CD163 Antibody (K20-T) NBP1-30148

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Protocols

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FAQs for CD163 Antibody (K20-T)

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  • Q: Can you please tell me antigen retrieval methods used for this antibody?

    A: For this product, our lab recommends HIER with (10 mM Tris/1 mM EDTA pH 9.0 solution). The protocol is detailed below: Pre-heat steamer or water bath with staining dish containing Tris/EDTA solution until temperature reaches 95-100 degrees Celsius. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

  • Q: Can you please tell me antigen retrieval methods used for this product (citrate or EDTA) NBP1-30148?

    A: For this product, our lab recommends HIER with (10 mM Tris/1 mM EDTA pH 9.0 solution). Protocol is detailed below:  Pre-heat steamer or water bath with staining dish containing Tris/EDTA solution until temperature reaches 95-100 degrees Celsius. Immerse slides in the staining dish.  Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

  • Q: Can you please tell me antigen retrieval methods used for this antibody?

    A: For this product, our lab recommends HIER with (10 mM Tris/1 mM EDTA pH 9.0 solution). The protocol is detailed below: Pre-heat steamer or water bath with staining dish containing Tris/EDTA solution until temperature reaches 95-100 degrees Celsius. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

  • Q: Can you please tell me antigen retrieval methods used for this product (citrate or EDTA) NBP1-30148?

    A: For this product, our lab recommends HIER with (10 mM Tris/1 mM EDTA pH 9.0 solution). Protocol is detailed below:  Pre-heat steamer or water bath with staining dish containing Tris/EDTA solution until temperature reaches 95-100 degrees Celsius. Immerse slides in the staining dish.  Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

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