CD31/PECAM-1 Antibody (C31.3)

Novus Biologicals | Catalog # NBP2-15202

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C31.3
View all CD31/PECAM-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CD31/PECAM-1 protein (Uniprot: P16284)

Reactivity Notes

Weakly reacts with Rat.

Localization

Cell surface and cytoplasm of endothelial cells

Marker

Endothelial Cell Marker

Specificity

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 monoclonal antibody reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33154)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD31/PECAM-1 Antibody (C31.3)

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using Azide/BSA FREE version of CD31/PECAM-1 Antibody (C31.3). Formalin-paraffin human Angiosarcoma.
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using the Azide and BSA Free version of CD31/PECAM-1 Antibody (C31.3). Staining of human tonsil.
Western Blot: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Western Blot: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Western Blot: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.3).
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with CD31/PECAM-1 Antibody (C31.3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)
Flow Cytometry: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Flow Cytometry: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202]

Flow Cytometry: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Flow Cytometric analysis of human CD31 on Jurkat cells. Black: cells alone; grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.3).
CD31/PECAM-1 Antibody (C31.3)

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] -

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Staining with CD31/PECAM-1 Antibody (C31.3) [Alexa Fluor® 647] (NBP2-33154AF647) using GE Cell DIVE (20x) on Human Rheumatoid Arthritis synovium (10 ug/ml). Image from verified customer review.
CD31/PECAM-1 Antibody (C31.3)

Immunohistochemistry: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] -

PFKFB3 expression is increased in advanced atherosclerotic plaques and correlates with CD68+ macrophages and necrotic core area. (A,B) Increased expression of PFKFB3 (green) in atheromatous plaques compared with fibrous carotid plaques. n = 10 for fibrous and fibroatheromatous plaques, n = 8 for atheromatous plaques; one-way ANOVA, P = 0.0078 for fibrous vs. atheromatous plaques. Scale bar 50 μm. (A,C) CD68+ macrophages (red) increased in atheromatous carotid plaques. n = 10 for fibrous and fibroatheromatous plaques, n = 8 for atheromatous plaques; one-way ANOVA, P = 0.0008 for fibrous vs. atheromatous plaques. Scale bar 50 μm (D) PFKFB3 expression and CD68 expression correlated significantly. n = 28; linear regression analysis, R2 = 0.7456, P < 0.0001. (E) Human coronary atherosclerotic advanced plaques showed increased PFKFB3 expression compared to initial plaques. n = 4 for initial vs. n = 5 for advanced plaques; two-tailed unpaired Mann-Whitney, P = 0.0317. Scale bar 200 μm. (F) PFKFB3 expression positively correlated with necrotic core area. n = 9; Linear regression analysis R2 = 0.4861, P = 0.0368. (G) Immunohistochemistry of PFKFB3+ co-staining with CD68+ macrophages; Scale bar 100 μm, (H) PECAM+ endothelial cells; Scale bar 100 μm, (I) CD3+ T cells; Scale bar 100 μm, and (J) and alpha smooth muscle actin ( alpha SMA)+ vascular smooth muscle cells (K); Scale bar 100 μm. Data are shown as mean +/- standard error of the mean. *P < 0.05, **P ≤ 0.005, ***P < 0.0005. PFKFB3, 6-phophofructo-2-kinase/fructose-2,6-biphosphatase 3; PECAM, platelet endothelial cell adhesion molecule; alpha SMA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33282864), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CD31/PECAM-1 Antibody (C31.3)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

0.5-1ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Gene Symbol

PECAM1

UniProt

P16284

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (C31.3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD31/PECAM-1 Antibody (C31.3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD31/PECAM-1 Antibody (C31.3)

Customer Reviews for CD31/PECAM-1 Antibody (C31.3)

There are currently no reviews for this product. Be the first to review CD31/PECAM-1 Antibody (C31.3) and earn rewards!

Have you used CD31/PECAM-1 Antibody (C31.3)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies