CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

Novus Biologicals | Catalog # NB600-1475

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, In vivo assay, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vivo assay, IF/IHC, In-vitro

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # MEC13.3

Format

BSA Free
View all CD31/PECAM-1 Primary Antibodies »

Product Specifications

Immunogen

This CD31/PECAM-1 Antibody (MEC13.3) was developed against mouse endothelial cell line T-end.

Reactivity Notes

Mouse (PMID: 7956830) and Human (PMID: 30626719) reactivity reported in scientific literature.

Localization

Membrane; Single-pass type I membrane protein. Cell junction.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC13.3] (NB600-1475) at 1ug/ml overnight at 4C and detected with an anti-rat DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - 4T1 tumor sections were stained for CD31 (green) and CD105 (red).
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).
Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - C57BL/6 mouse splenocytes stained with purified CD31/PECAM-1 Antibody (MEC13.3), followed by anti-rat IgG FITC
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC13.3] conjugated to DyLight 488 (NB600-1475G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with at 5.0 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - was tested in Wehi-3 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

CD31-PECAM-1-Antibody-MEC13-3-Immunohistochemistry-Paraffin-NB600-1475-img0015.jpg
Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3)BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3)BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC13.3] conjugated to Alexa Fluor 647 (NB600-1475AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD31/PECAM-1 Antibody [MEC13.3] conjugated to DyLight 550 (NB600-1475R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - Staining of mouse CD31 in non-small cell lung cancer tumor sections from a human NSCLC tumor-bearing mouse. This image was submitted via customer Review.
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on WEHI-3 Cells with CD31 (MEC13.3) antibody NB600-1475 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems).
Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS-1 cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475AF647, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3)BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3)BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475DL594 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 594.
Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3)BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3)BSA Free [NB600-1475]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] - A surface stain was performed on MS1 cells with CD31/PECAM-1 [MEC13.3] Antibody NB600-1475G (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.
CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475] -

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - BSA Free [NB600-1475] - (A) Representative Western blots depicting phosphorylated JAK3 (pJAK3), total JAK3, & corresponding beta -actin levels in the ipsilateral (CXI) versus the contralateral (CXC) cortex at 24 h in sham & mice subjected to permanent middle cerebral artery occlusion (pMCAO). (B,C) Quantified pJAK3 & JAK3 values normalized to beta -actin in the cortex of sham & mice subjected to stroke. Both pJAK3 & JAK3 are significantly increased in the ipsilateral cortex of stroked animals (*P < 0.05 & **P < 0.01 with respect to sham ipsilateral/contralateral & stroke contralateral; sham, n = 5; stroke, n = 13). Data were analyzed using a two-way ANOVA (ipsilateral/contralateral or sham/stroke) with Bonferroni post-test. (D) Diagram showing peri-infarct brain region from which images were taken (n = 3). Scale bar is 10 µm, & magnification is 60×. (E) JAK3 colocalized with microglia/macrophages labeled with Iba-1. (F) JAK3 colocalized with neurons labeled with NeuN. (G) JAK3 colocalized with endothelial cells labeled with CD31. (H) JAK3 is not colocalized with astrocytes labeled with GFAP. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28790974), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

CD31/PECAM-1 (MEC13.3) in MS1 Mouse Cell Line.

CD31/PECAM-1 (MEC13.3) was detected in immersion fixed MS1 mouse pancreas/Islet of Langerhans endothelial cell line using Rat anti-CD31/PECAM-1 (MEC13.3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NB600-1475R) (red) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

2.5 ug/ml

Flow Cytometry

2.5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:500 - 1:1000

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:100

Immunoprecipitation

1:10-1:500

In vitro assay

reported in scientific literature (PMID 24647208)

In vivo assay

reported in scientific literature
Application Notes
This CD31/PECAM1 Antibody (MEC13.3) is useful for in vitro and in vivo blocking of CD31-mediated cell-cell interactions. Please Note: NB600-1475 works well on acetone-fixed frozen sections as well as zinc-fixed paraffin-embedded sections. However, inconsistent results were observed for formalin-fixed paraffin-embedded sections. This antibody is CyTOF ready.

Reviewed Applications

Read 2 reviews rated 5 using NB600-1475 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1, MEC 13.3 CD31, MEC 13.3 Clone

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

Customer Reviews for CD31/PECAM-1 Antibody (MEC13.3) - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • CD31/PECAM-1 Antibody (MEC13.3)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Non-small cell lung cancer
    Species: Human
    Verified Customer | Posted 08/29/2017
    IHC staining of mouse CD31 in tumor sections from a human NSCLC tumor-bearing mouse
    CD31/PECAM-1 Antibody (MEC13.3) - BSA Free NB600-1475
  • CD31/PECAM-1 Antibody (MEC13.3)
    Name: Christopher England
    Application: Immunohistochemistry-Frozen
    Sample Tested: 4T1 tissue
    Species: Mouse
    Verified Customer | Posted 02/24/2017
    4T1 tumor sections were stained for CD31 (green) and CD105 (red).
    Green is the CD31, which looked perfect!
    CD31/PECAM-1 Antibody (MEC13.3) - BSA Free NB600-1475

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Protocols

View specific protocols for CD31/PECAM-1 Antibody (MEC13.3) - BSA Free (NB600-1475):

Protocol for Flow Cytometry Cell Surface Staining
Sample Preparation.
1. Grow cells to 60-85% confluency. Flow cytometry requires between 2 x 105 and 1 x 106 cells for optimal performance.
2. If cells are adherent, harvest gently by washing once with staining buffer and then scraping. Avoid using trypsin as this can disrupt certain epitopes of interest. If enzymatic harvest is required, use Accutase, Collagenase, or TrypLE Express for a less damaging option.
3. Reserve 100 uL for counting, then transfer cell volume into a 15 mL conical tube and centrifuge for 4 minutes at 400 RCF.
a. Count cells using a hemocytometer and a 1:1 trypan blue exclusion stain to determine cell viability before starting the flow protocol. If cells appear blue, do not proceed.
4. Re-suspend cells to a concentration of 1 x 106 cells/mL in staining buffer (NBP2-26247).
5. Aliquot out 100 uL samples in accordance with your experimental samples.

Tip: When cell surface and intracellular staining are required in the same sample, it is advisable that the cell surface staining be performed first since the fixation and permeablization steps might reduce the availability of surface antigens.
Cell surface staining
1. Recommended: Block non-specific interactions using 0.5-1 ug of a species specific Fc-blocking reagent such as an anti-mouse CD16/CD32 antibody (NBP1-27946).
2. Add appropriate amount of each antibody (eg. 1 test or 1 ug per sample, as experimentally determined) to 100 uL of staining buffer (NBP2-26247) per sample (eg. use 1 mL of staining buffer for 10 samples).
3. Mix well and incubate at room temperature in dark for 20 minutes.
4. Add 1-2 mL of staining buffer and centrifuge at 400 RCF for 1 minute and discard supernatant.
5. Wash twice by re-suspending cells in staining buffer (2 mL for tubes or 200 uL for wells) and centrifuging at 400 RCF for 5 minutes. Discard supernatant.
6. Add appropriate amount of secondary antibody (as experimentally determined) to each sample.
7. Incubate at room temperature in dark for 20 minutes.
8. Add 1-2 mL of staining buffer and centrifuge at 400 RCF for 1 minute and discard supernatant.
9. Wash twice by re-suspending cells in staining buffer (2 mL for tubes or 200 uL for wells) and centrifuging at 400 RCF for 5 minutes. Discard supernatant.
10. Resuspend in an appropriate volume of staining buffer (usually 500 uL per sample) and proceed with analysis on your flow cytometer.

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 10% formalin to the dish and fix at room temperature for 10 minutes.
2. Remove the formalin and wash the cells in PBS.
3. Permeablize the cells with 0.1% Triton X100 or other suitable detergent for 10 min.
4. Remove the permeablization buffer and wash three times for 10 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 10 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 10 minutes each.
10. Counter stain DNA with DAPi if required.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] was left out at room temperature for 3 days and is not working. Do you think the antibody is degraded?

    A: This antibody is supplied in PBS with 0.05% Sodium Azide and we suggest storing this antibody at 4C while small aliqots can be stored at -20C also. When in lyophillized form, the antibodies can stay stable to some extent at room temperature but in liquid form, they often degrade and thats probably what happened in your case also. We suggest ordering a new stock of antibody so you do not have to waste your valuable time & resources by trying the non-working or degraded antibody. 

  • Q: I like to know that CD31/PECAM1 Antibody (MEC13.3) (NB600-1475), will work with NBF fixed paraffin embedded sample, not zinc fixed.

    A: It looks like NB600-1475 can be used in IHC of acetone-fixed frozen sections and zinc-fixed paraffin embedded sections. However, it is not recommended for formalin-fixed paraffin-embedded sections due to inconsistent results.

  • Q: CD31/PECAM-1 Antibody (MEC13.3) [NB600-1475] was left out at room temperature for 3 days and is not working. Do you think the antibody is degraded?

    A: This antibody is supplied in PBS with 0.05% Sodium Azide and we suggest storing this antibody at 4C while small aliqots can be stored at -20C also. When in lyophillized form, the antibodies can stay stable to some extent at room temperature but in liquid form, they often degrade and thats probably what happened in your case also. We suggest ordering a new stock of antibody so you do not have to waste your valuable time & resources by trying the non-working or degraded antibody. 

  • Q: I like to know that CD31/PECAM1 Antibody (MEC13.3) (NB600-1475), will work with NBF fixed paraffin embedded sample, not zinc fixed.

    A: It looks like NB600-1475 can be used in IHC of acetone-fixed frozen sections and zinc-fixed paraffin embedded sections. However, it is not recommended for formalin-fixed paraffin-embedded sections due to inconsistent results.

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