CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80640

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, In vivo assay, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # MEC13.3

Format

Azide and BSA Free
View all CD31/PECAM-1 Primary Antibodies »

Product Specifications

Immunogen

This CD31/PECAM-1 Antibody (MEC13.3) was developed against mouse endothelial cell line T-end.

Reactivity Notes

Mouse and Human reactivity reported in scientific literature.

Localization

Membrane; Single-pass type I membrane protein. Cell junction.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free

Immunocytochemistry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunocytochemistry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunocytochemistry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640] - Staining of mouse CD31 in non-small cell lung cancer tumor sections from a human NSCLC tumor-bearing mouse. This image was submitted via customer Review. Image from the standard format of this antibody.
Immunohistochemistry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunohistochemistry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunohistochemistry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640] - JAK3 colocalized with endothelial cells labeled with CD31. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fneur.2017.00363/full), licensed under a CC-BY license. Image from the standard
Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640] - A surface stain was performed on MS-1 cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475AF647, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640] - CD31/PECAM-1 Antibody (MEC13.3) was tested in Wehi-3 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640] - MS1 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with CD31/PECAM-1 Antibody (MEC13.3) at 5.0 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunohistochemistry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Immunohistochemistry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640] - 4T1 tumor sections were stained for CD31 (green) and CD105 (red). Image from the standard format of this antibody.
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640] - A surface stain was performed on WEHI-3 Cells with CD31 (MEC13.3) antibody NB600-1475 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems). Image from the standard format of this antibody.
Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Flow (Cell Surface): CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640] - A surface stain was performed on WEHI-3 Cells with CD31/PECAM-1 Antibody (MEC13.3) (NB600-1475, blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature, followed by rat F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0113, R&D Systems). Image from the standard format of this antibody.
Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640]

Flow Cytometry: CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free [NBP2-80640] - C57BL/6 mouse splenocytes stained with purified CD31/PECAM-1 Antibody (MEC13.3), followed by anti-rat IgG FITC Image from the standard format of this antibody.

Applications for CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:1000-1:2000

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1:10-1:500

In vitro assay

reported in scientific literature (PMID 24647208)

In vivo assay

reported in scientific literature
Application Notes
This CD31/PECAM1 Antibody (MEC13.3) is useful for in vitro and in vivo blocking of CD31-mediated cell-cell interactions. Please Note: NB600-1475 works well on acetone-fixed frozen sections as well as zinc-fixed paraffin-embedded sections. However, it is not recommended for formalin-fixed paraffin-embedded sections due to inconsistent results. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free

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Product Specific Notices for CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD31/PECAM-1 Antibody (MEC13.3) - Azide and BSA Free

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