CD40/TNFRSF5 Antibody (CL1673) - BSA Free

Novus Biologicals | Catalog # NBP2-34488

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1673

Format

BSA Free
View all CD40/TNFRSF5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P25942, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD40/TNFRSF5 Antibody (CL1673) - BSA Free

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining in human tonsil and cerebral cortex tissues. Corresponding CD40 RNA-seq data are presented for the same tissues.
Western Blot: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Western Blot: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Western Blot: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Lane 1: Marker [kDa] Lane 2: Human spleen
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Human stomach cancer FFPE block stained with CD40 antibody at 1:6000 overnight. Antigen retrieval at pH 9. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining of human tonsil shows strong membranous positivity in germinal center cells.
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining of human colon shows moderate membranous positivity in lymphoid cells.
Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (CL1673) [NBP2-34488] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.

Applications for CD40/TNFRSF5 Antibody (CL1673) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP2-34488 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD40/TNFRSF5

CD40 and its ligand CD154 are members of the tumor necrosis factor receptor (TNFR) and TNF families, respectively, that play key roles in signaling pathways mediating cell growth, survival and differentiation in B-lymphocytes (reviewed in Quezada et al 2004). The CD40 receptor is a 45-50 kDa glycoprotein and is expressed on the surface of B-lymphocytes, some activated T-cells, monocytes, follicular dendritic cells, basal epithelial cells, and in some epithelial and non-epithelial carcinomas. The functions of CD40 have been most extensively studied in B-cells. Ligation of B CD40 by CD154, expressed on activated T cells, stimulates B cell proliferation, differentiation, isotype switching, upregulation of surface molecules contributing to antigen presentation, development of the germinal center, and the humoral memory response. Several distinct structural motifs in the CD40 cytoplasmic domain regaulate various CD40 signaling pathways. A major CD40 signaling pathway activated from CD154 ligand binding is the canonical pathway to the transcription factor family NF-kB, a family of genes mediating immune and inflammatory responses. Although CD40 has been extensively studied as a plasma membrane-associated growth factor membrane receptor. it has also been identified in the cytoplasm and nucleus of normal and neoplastic B-lymphoid cells (Lin-Lee et al. 2006). Other growth factor receptors, including EGF, FGF, and TGF-B have also been identified in the nuclus. It is thought that plasma membrane receptor signaling may be followed by nuclear migration of signaling pathway components. The presence of CD40 in the nucleus of activated normal B lymphocytes and neoplastic B-lymphoid cells suggests that CD40 may play a more complex role in regulating essential growth and survival pathways in B-lymphocytes than previously thought.

Alternate Names

CD40, TNFRSF5

Gene Symbol

CD40

UniProt

P25942

Additional CD40/TNFRSF5 Products

Product Documents for CD40/TNFRSF5 Antibody (CL1673) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD40/TNFRSF5 Antibody (CL1673) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD40/TNFRSF5 Antibody (CL1673) - BSA Free

Customer Reviews for CD40/TNFRSF5 Antibody (CL1673) - BSA Free (1)

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Customer Images

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  • CD40/TNFRSF5 Antibody (CL1673)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: FFPE stomach cancer
    Species: Human
    Verified Customer | Posted 03/30/2021
    FFPE block of stomach cancer stained with anti-CD40 O/N 1in6000
    Antigen Retrieval ph9, Incubation Overnight, 1in6000
    CD40/TNFRSF5 Antibody (CL1673) - BSA Free NBP2-34488

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Protocols

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FAQs for CD40/TNFRSF5 Antibody (CL1673) - BSA Free

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  • Q: I wanna know does CD40 antibody for IHC-P comes with positive control slide?

    A: Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.

  • Q: We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?

    A: Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

  • Q: I wanna know does CD40 antibody for IHC-P comes with positive control slide?

    A: Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.

  • Q: We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?

    A: Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

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