CD40/TNFRSF5 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56127

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CD40/TNFRSF5 Primary Antibodies »

Product Specifications

Immunogen

A recombinant protein containing the short cytoplasmic domain of the human CD40 molecule was used as immunogen.

Reactivity Notes

Cross reacts with Gerbil.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD40/TNFRSF5 Antibody - BSA Free

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody [NB100-56127]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody [NB100-56127]

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody [NB100-56127] - FFPE tissue section of human tonsil stained for CD40 expression using this antibody at 1:2000. Staining is seen in the dendritic cells. Hematoxylin-eosin counterstain.
CD40/TNFRSF5 Antibody - BSA Free

Western Blot: CD40/TNFRSF5 Antibody - BSA Free [NB100-56127] -

The contribution of oligodendrocytes to the BDEVs pool is significantly upregulated at 72 h after tMCAO. A Western blots of sBDEVs samples from tMCAO and shams (n = 5 per group) blotted for cell-type-specific markers: PLP and CNP1 are used as protein markers for oligodendrocytes (orange frame); synapsin 1 (Syn1) and NCAM as markers for neurons (green); EAAT1 and EAAT2 as protein markers for astrocytes (pink) and P2Y12 and CD40 as markers for microglia/macrophages (blue). TH is a total mouse brain homogenate loaded in parallel for comparison purposes. TS is a representative total protein staining of the nitrocellulose membranes (TSs of all blots used for these analyses are provided in Suppl. Fig. 5). B Dot plots showing the quantifications of the western blot intensities. For the quantification, each band intensity was first referred to the corresponding lanes of the total protein staining. Both markers for oligodendrocytes were found significantly increased upon stroke. Regarding neuronal markers, NCAM was significantly increased while Syn1 only showed a tendency to be elevated. Exact p-values are given in the main text Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35639208), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CD40/TNFRSF5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

1:50 - 1:200

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD40/TNFRSF5

CD40 and its ligand CD154 are members of the tumor necrosis factor receptor (TNFR) and TNF families, respectively, that play key roles in signaling pathways mediating cell growth, survival and differentiation in B-lymphocytes (reviewed in Quezada et al 2004). The CD40 receptor is a 45-50 kDa glycoprotein and is expressed on the surface of B-lymphocytes, some activated T-cells, monocytes, follicular dendritic cells, basal epithelial cells, and in some epithelial and non-epithelial carcinomas. The functions of CD40 have been most extensively studied in B-cells. Ligation of B CD40 by CD154, expressed on activated T cells, stimulates B cell proliferation, differentiation, isotype switching, upregulation of surface molecules contributing to antigen presentation, development of the germinal center, and the humoral memory response. Several distinct structural motifs in the CD40 cytoplasmic domain regaulate various CD40 signaling pathways. A major CD40 signaling pathway activated from CD154 ligand binding is the canonical pathway to the transcription factor family NF-kB, a family of genes mediating immune and inflammatory responses. Although CD40 has been extensively studied as a plasma membrane-associated growth factor membrane receptor. It has also been identified in the cytoplasm and nucleus of normal and neoplastic B-lymphoid cells (Lin-Lee et al. 2006). Other growth factor receptors, including EGF, FGF, and TGF-B have also been identified in the nuclus. It is thought that plasma membrane receptor signaling may be followed by nuclear migration of signaling pathway components. The presence of CD40 in the nucleus of activated normal B lymphocytes and neoplastic B-lymphoid cells suggests that CD40 may play a more complex role in regulating essential growth and survival pathways in B-lymphocytes than previously thought.

Alternate Names

CD40, TNFRSF5

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat)

Gene Symbol

CD40

Additional CD40/TNFRSF5 Products

Product Documents for CD40/TNFRSF5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CD40/TNFRSF5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD40/TNFRSF5 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD40/TNFRSF5 Antibody - BSA Free

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  • Q: I wanna know does CD40 antibody for IHC-P comes with positive control slide?

    A: Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.

  • Q: We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?

    A: Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

  • Q: I wanna know does CD40 antibody for IHC-P comes with positive control slide?

    A: Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.

  • Q: We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?

    A: Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

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