CD43/Sialophorin Antibody (SPN/839)

Novus Biologicals | Catalog # NBP2-44648

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPN/839
View all CD43 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CD43/Sialophorin protein (Uniprot: P16150)

Localization

Cell surface

Marker

T-Cell Marker

Specificity

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47879)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD43/Sialophorin Antibody (SPN/839)

Western Blot: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Western Blot: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Western Blot: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Western Blot Analysis of K562 cell lysate using CD43/Sialophorin Antibody (SPN/839).
Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (SPN/839) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot Live 650.
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Human Spleen stained with CD43 Monoclonal Antibody (SPN/839).
Flow Cytometry: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Flow Cytometry: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Flow Cytometry: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Flow Cytometric Analysis of K562 cells using CD43/Sialophorin Antibody (SPN/839) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Western Blot: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648]

Western Blot: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Analysis of human Spleen Lysate using CD43 Monoclonal Antibody (SPN/839).

Applications for CD43/Sialophorin Antibody (SPN/839)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD43

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Gene Symbol

SPN

UniProt

P16150

Additional CD43 Products

Product Documents for CD43/Sialophorin Antibody (SPN/839)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD43/Sialophorin Antibody (SPN/839)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD43/Sialophorin Antibody (SPN/839)

There are currently no reviews for this product. Be the first to review CD43/Sialophorin Antibody (SPN/839) and earn rewards!

Have you used CD43/Sialophorin Antibody (SPN/839)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies