CD44 Antibody (9A4) - variant 6 - BSA Free

Novus Biologicals | Catalog # NB100-64818

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry

Cited:

Western Blot, Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 9A4

Format

BSA Free
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

GST-CD44v6 fusion protein

Marker

Cell membrane Marker

Specificity

Recognizes an epitope encoded by exon v6 on the variant portion of murine CD44.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for CD44 Antibody (9A4) - variant 6 - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Use in FLOW reported in scientific literature (PMID: 30504836).

Flow Cytometry Panel Builder

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

12505 (Mouse)

Gene Symbol

CD44

UniProt

P15379 (Mouse)

Additional CD44 Products

Product Documents for CD44 Antibody (9A4) - variant 6 - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD44 Antibody (9A4) - variant 6 - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD44 Antibody (9A4) - variant 6 - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD44 Antibody (9A4) - variant 6 - BSA Free

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  • Q: We used the CD44 variant 6 antibody (9A4, NB100-64818) in our Lab. I did not find the molecular weight of this antibody on the data sheet or in the related literature. I would appreciate it if you could give me some information about the molecular weight of this antibody.

    A: The lab has informed me that on testing of the immunogen the protein is expressed at 80-85 kDa, but when tested on endogenous samples the protein is picked up at approx. 200 kDa. Most likely the protein forms a dimer, or complex when picked up in endogenous samples. Additionally, I found a paper, PMC1853452 (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1853452/), that shows CD44v6 should be expressed at approx. 200 kDa - 250 kDa.

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